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פותח על ידי קלירמאש פתרונות בע"מ -
Cyclic AMP mediation of the hormonal stimulation of 14C-acetate incorporation by Heliothis armigera pheromone glands in vitro
Year:
1989
Authors :
סורוקר, ויקטוריה
;
.
רפאלי, עדה
;
.
Volume :
65
Co-Authors:
Rafaeli, A., Department of Entomology, Faculty of Agriculture, 76-100 Rehovot, Israel
Soroker, V., Department of Entomology, Faculty of Agriculture, 76-100 Rehovot, Israel
Facilitators :
From page:
43
To page:
48
(
Total pages:
6
)
Abstract:
Evidence is presented for hormone-controlled adenosine 3',5'-cyclic monophosphate (cAMP)-mediated incorporation of 14C-acetate by in vitro pheromone gland preparations of Heliothis armigera. Basal intracellular cAMP concentrations of in vitro pheromone glands increase 28-fold in the presence of methanolic brain-complex extracts after 5 min of incubation. This intracellular cAMP increase shows a dose-dependent response. Studies on isolated pheromone gland preparations show that 8-bromo-cAMP added to the incubation media stimulates acetate incorporation thereby mimicking the hormonal response. This effect was duplicated by forskolin and isobutyl-methylxanthine. In addition, forskolin exhibits a dose-dependent stimulatory effect on the incorporation of 14C-acetate by such isolated in vitro pheromone glands. High-pressure liquid Chromatographic analysis of the radioactive products, extracted by hexane from pheromone glands and media, revealed a stimulation by forskolin of a product exhibiting the same retention time as (Z)-11-hexadecenal, the main pheromone component of H. armigera. In addition, thin-layer Chromatographic analysis revealed co-elution with (Z)-11-hexadecenal on stimulation by 8-bromo-cAMP. © 1989.
Note:
Related Files :
Animal
Chromatography, High Pressure Liquid
Cyclic AMP
Female
Lepidoptera
pheromones
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0303-7207(89)90163-9
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31679
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:04
You may also be interested in
Scientific Publication
Cyclic AMP mediation of the hormonal stimulation of 14C-acetate incorporation by Heliothis armigera pheromone glands in vitro
65
Rafaeli, A., Department of Entomology, Faculty of Agriculture, 76-100 Rehovot, Israel
Soroker, V., Department of Entomology, Faculty of Agriculture, 76-100 Rehovot, Israel
Cyclic AMP mediation of the hormonal stimulation of 14C-acetate incorporation by Heliothis armigera pheromone glands in vitro
Evidence is presented for hormone-controlled adenosine 3',5'-cyclic monophosphate (cAMP)-mediated incorporation of 14C-acetate by in vitro pheromone gland preparations of Heliothis armigera. Basal intracellular cAMP concentrations of in vitro pheromone glands increase 28-fold in the presence of methanolic brain-complex extracts after 5 min of incubation. This intracellular cAMP increase shows a dose-dependent response. Studies on isolated pheromone gland preparations show that 8-bromo-cAMP added to the incubation media stimulates acetate incorporation thereby mimicking the hormonal response. This effect was duplicated by forskolin and isobutyl-methylxanthine. In addition, forskolin exhibits a dose-dependent stimulatory effect on the incorporation of 14C-acetate by such isolated in vitro pheromone glands. High-pressure liquid Chromatographic analysis of the radioactive products, extracted by hexane from pheromone glands and media, revealed a stimulation by forskolin of a product exhibiting the same retention time as (Z)-11-hexadecenal, the main pheromone component of H. armigera. In addition, thin-layer Chromatographic analysis revealed co-elution with (Z)-11-hexadecenal on stimulation by 8-bromo-cAMP. © 1989.
Scientific Publication
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