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פותח על ידי קלירמאש פתרונות בע"מ -
DNA fingerprinting of strawberry (Fragaria x ananassa) cultivars using randomly amplified polymorphic DNA (RAPD) markers
Year:
1998
Source of publication :
Euphytica
Authors :
דגני, חמדה
;
.
Volume :
102
Co-Authors:
Degani, C., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan 50250, Israel
Rowland, L.J., Fruit Laboratory, Beltsville Agric. Research Center, Agriculture Research Service, Beltsville, MD 20705, United States
Levi, A., Fruit Laboratory, Beltsville Agric. Research Center, Agriculture Research Service, Beltsville, MD 20705, United States
Hortynski, J.A., Dept. Genet. and Hort. Plant Breed., University of Agriculture, Akademicka 15 St., 20-950 Lublin, Poland
Galletta, G.J., Fruit Laboratory, Beltsville Agric. Research Center, Agriculture Research Service, Beltsville, MD 20705, United States
Facilitators :
From page:
247
To page:
253
(
Total pages:
7
)
Abstract:
Forty-one of the major strawberry (Fragaria x ananassa Duch.) cultivars grown in the United States and Canada were examined for RAPD (randomly amplified polymorphic DNA) marker polymorphisms using 10mer primers (> 50% GC content). A set of 10 primers produced 15 polymorphic fragments ranging in size between 450 and 1200 bp, which were more than sufficient to distinguish among all tested cultivars. Ten of the markers derived from seven primers were absolutely required for distinguishing the cultivars. A DNA fingerprinting table was constructed based on these results. In addition, similarity coefficients were calculated based on RAPD marker data and a dendogram was constructed using the unweighted pair group method of arithmetic averages (UPGMA). These results were compared with known pedigree data for the cultivars. Our results demonstrate that RAPD markers can be used effectively for strawberry cultivar identification.
Note:
Related Files :
Canada
cultivar identification
Fragaria
Fragaria x ananassa
genetic diversity
RAPD markers
strawberry
United States
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31712
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:04
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Scientific Publication
DNA fingerprinting of strawberry (Fragaria x ananassa) cultivars using randomly amplified polymorphic DNA (RAPD) markers
102
Degani, C., Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet-Dagan 50250, Israel
Rowland, L.J., Fruit Laboratory, Beltsville Agric. Research Center, Agriculture Research Service, Beltsville, MD 20705, United States
Levi, A., Fruit Laboratory, Beltsville Agric. Research Center, Agriculture Research Service, Beltsville, MD 20705, United States
Hortynski, J.A., Dept. Genet. and Hort. Plant Breed., University of Agriculture, Akademicka 15 St., 20-950 Lublin, Poland
Galletta, G.J., Fruit Laboratory, Beltsville Agric. Research Center, Agriculture Research Service, Beltsville, MD 20705, United States
DNA fingerprinting of strawberry (Fragaria x ananassa) cultivars using randomly amplified polymorphic DNA (RAPD) markers
Forty-one of the major strawberry (Fragaria x ananassa Duch.) cultivars grown in the United States and Canada were examined for RAPD (randomly amplified polymorphic DNA) marker polymorphisms using 10mer primers (> 50% GC content). A set of 10 primers produced 15 polymorphic fragments ranging in size between 450 and 1200 bp, which were more than sufficient to distinguish among all tested cultivars. Ten of the markers derived from seven primers were absolutely required for distinguishing the cultivars. A DNA fingerprinting table was constructed based on these results. In addition, similarity coefficients were calculated based on RAPD marker data and a dendogram was constructed using the unweighted pair group method of arithmetic averages (UPGMA). These results were compared with known pedigree data for the cultivars. Our results demonstrate that RAPD markers can be used effectively for strawberry cultivar identification.
Scientific Publication
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