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Aquaculture (source)
Agresti, J.J., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States
Seki, S., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States, Department of Aquaculture, Fac. Agric., Kochi Univ., B-200, M., Kochi, Japan
Cnaani, A., Department of Aquaculture, Volcani Ctr., Agric. Res. Org., P.O., Bet Dagan, Israel
Poompuang, S., Department of Aquaculture, Fac. Fish., Kasetsart Univ., C., Bangkok, Thailand, Dept. of Fish. and Wildlife Sciences, Virginia Polytech. Inst. Stt. Univ., Blacksburg, VA 24061-0321, United States
Hallerman, E.M., Dept. of Fish. and Wildlife Sciences, Virginia Polytech. Inst. Stt. Univ., Blacksburg, VA 24061-0321, United States
Umiel, N., Dept. of Ornamental Horticulture, Volcani Ctr., Agric. Res. Org., P.O., Bet Dagan, Israel
Hulata, G., Department of Aquaculture, Volcani Ctr., Agric. Res. Org., P.O., Bet Dagan, Israel
Gall, G.A.E., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States
May, B., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States, Department of Natural Resources, Fernow Hall, Cornell University, Ithaca, NY 14853, United States
Based on ideas from plant breeding and the opportunities offered by molecular biology, a program was initiated in 1995 to derive genetically superior tilapia from a synthetic stock (artificial center of origin, ACO) produced by inter-crossing five groups of fish: Oreochromis niloticus [wild-type (On) and red (ROn) strains], O. aureus (Oa), O. mossambicus (Om), and Sarotherodon galilaeus (Sg). Three-way cross families (3WC) and four-way cross families (4WC) have been produced, so that all four species are represented in the ACO. A genomic map has been created for each of the parents in an Om X (Oa X ROn) family using microsatellite and AFLP (amplified fragment length polymorphism) DNA markers. The female (Om) parent had a total of 78 segregating markers (17 microsatellites, 61 AFLPs). Of these, 62 (13 microsatellites, 49 AFLPs) were linked in 14 linkage groups covering a total of 514 centimorgans (cM). The first generation (F1) hybrid male parent had a total of 229 segregating markers (62 microsatellites, 167 AFLPs), of which 214 (60 microsatellites, 154 AFLPs) were linked in 24 linkage groups covering a total of 1632 cM. The construction of these maps is a key step in a molecular marker-assisted breeding program to detect quantitative trait loci (QTL) for cold and salinity tolerance and carcass quality in tilapia. (C) 2000 Elsevier Science B.V.
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Breeding new strains of tilapia: Development of an artificial center of origin and linkage map based on AFLP and microsatellite loci
185
Agresti, J.J., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States
Seki, S., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States, Department of Aquaculture, Fac. Agric., Kochi Univ., B-200, M., Kochi, Japan
Cnaani, A., Department of Aquaculture, Volcani Ctr., Agric. Res. Org., P.O., Bet Dagan, Israel
Poompuang, S., Department of Aquaculture, Fac. Fish., Kasetsart Univ., C., Bangkok, Thailand, Dept. of Fish. and Wildlife Sciences, Virginia Polytech. Inst. Stt. Univ., Blacksburg, VA 24061-0321, United States
Hallerman, E.M., Dept. of Fish. and Wildlife Sciences, Virginia Polytech. Inst. Stt. Univ., Blacksburg, VA 24061-0321, United States
Umiel, N., Dept. of Ornamental Horticulture, Volcani Ctr., Agric. Res. Org., P.O., Bet Dagan, Israel
Hulata, G., Department of Aquaculture, Volcani Ctr., Agric. Res. Org., P.O., Bet Dagan, Israel
Gall, G.A.E., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States
May, B., Department of Animal Science, Meyer Hall, Univ. California, D., Davis, CA 95616, United States, Department of Natural Resources, Fernow Hall, Cornell University, Ithaca, NY 14853, United States
Breeding new strains of tilapia: Development of an artificial center of origin and linkage map based on AFLP and microsatellite loci
Based on ideas from plant breeding and the opportunities offered by molecular biology, a program was initiated in 1995 to derive genetically superior tilapia from a synthetic stock (artificial center of origin, ACO) produced by inter-crossing five groups of fish: Oreochromis niloticus [wild-type (On) and red (ROn) strains], O. aureus (Oa), O. mossambicus (Om), and Sarotherodon galilaeus (Sg). Three-way cross families (3WC) and four-way cross families (4WC) have been produced, so that all four species are represented in the ACO. A genomic map has been created for each of the parents in an Om X (Oa X ROn) family using microsatellite and AFLP (amplified fragment length polymorphism) DNA markers. The female (Om) parent had a total of 78 segregating markers (17 microsatellites, 61 AFLPs). Of these, 62 (13 microsatellites, 49 AFLPs) were linked in 14 linkage groups covering a total of 514 centimorgans (cM). The first generation (F1) hybrid male parent had a total of 229 segregating markers (62 microsatellites, 167 AFLPs), of which 214 (60 microsatellites, 154 AFLPs) were linked in 24 linkage groups covering a total of 1632 cM. The construction of these maps is a key step in a molecular marker-assisted breeding program to detect quantitative trait loci (QTL) for cold and salinity tolerance and carcass quality in tilapia. (C) 2000 Elsevier Science B.V.
Scientific Publication
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