Co-Authors:
Hatew, B., Dept. of Food Science, Agricultural Research Organization (ARO), Volcani Center, P.O. Box, Bet-Degan 50250, Israel, Animal Nutrition Group, Wageningen Univ., Marijkeweg 40, 6709 PG, Wageningen, Netherlands
Delessa, T., Dept. of Food Science, Agricultural Research Organization (ARO), Volcani Center, P.O. Box, Bet-Degan 50250, Israel, Inst. of Food, Nutrition and Health, Swiss Federal Inst. of Technology Zurich (ETH), Schorenstrasse 16, 8603, Schwerzenbach, Switzerland
Zakin, V., Dept. of Food Science, Agricultural Research Organization (ARO), Volcani Center, P.O. Box, Bet-Degan 50250, Israel
Gollop, N., Dept. of Food Science, Agricultural Research Organization (ARO), Volcani Center, P.O. Box, Bet-Degan 50250, Israel
Abstract:
Chicken intestine harbors a vast number of bacterial strains. In the present study, antimicrobial substance produced by lactic acid bacteria (LAB) isolated from the gastrointestinal tract of healthy chicken was detected, characterized, and purified. Based on 16S rRNA sequencing, the bacteria were identified as Lactobacillus plantarum vN. The antimicrobial substance produced by this bacterium was designated vN-1 and exhibited a broad-spectrum of activity against many important pathogenic and spoilage microorganisms, including Pseudomonas aeruginosa, Staphylococcus aureus, Micrococcus luteus, Salmonella Typhimurium, and Erwinia amylovova. vN-1 was determined to be thermostable, insensitive to pH values ranging from 2.0 to 8.0, resistant to various organic solvents and to enzymatic inactivation. The inhibition kinetics displayed a bactericidal mode of action. This study revealed an antimicrobial substance with low molecular mass of less than 1 kDa as determined by ultrafiltration and having features not previously reported for LAB isolated from chicken intestines. The detection of this antimicrobial substance addresses an important aspect of biotechnological control agents of spoilage caused by Pseudomonas spp. and promises the possibility for preservation of refrigerated poultry meat. Practical Application: The newly characterized antimicrobial substance and designated as vN-1 may have the potential to be used in food preservation. © 2011 Institute of Food Technologists ®.
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מונוכרום
הדגשת קישורים
חסימת אנימציה
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