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פותח על ידי קלירמאש פתרונות בע"מ -
Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells
Year:
2000
Source of publication :
FEMS Microbiology Letters
Authors :
סלע, שלמה
;
.
Volume :
193
Co-Authors:
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel
Marouni, M.J., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel
Perry, R., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel
Barzilai, A., Pediatric Infectious Diseases Unit, Chaim Sheba Medical Center, Tel-Hashomer Hospital, Tel-Hashomer, Israel
Facilitators :
From page:
187
To page:
193
(
Total pages:
7
)
Abstract:
Lipoteichoic acid (LTA) is thought to play a role in the interactions between Streptococcus pyogenes and host cells. We have examined the effect of exogenous LTA on the adherence and entry of S. pyogenes JRS4 strain into HEp-2 epithelial cells. LTA markedly inhibited bacterial entry in a concentration-dependent manner, up to 250 μg ml-1. In contrast, LTA had only a slight inhibitory effect on adherence. LTA also inhibited the entry but not adherence of Salmonella typhimurium strain into HEp-2 cells. Binding experiments showed a dose-dependent binding of LTA to cells up to 10 μg ml-1. Confocal laser microscopy imaging and analysis revealed that LTA was internalized by the epithelial cells and colocalized with F-actin. These results might imply that, following binding, exogenous LTA enters HEp-2 cells and exerts a cytotoxic effect that interferes with bacterial internalization. A possible target for LTA activity might be the actin cytoskeleton, which is known to be essential for bacterial uptake. Copyright (C) 2000 Federation of European Microbiological Societies.
Note:
Related Files :
confocal laser microscopy
Group A streptococcus
human cell
Lipoteichoic acid
Streptococcus sp. 'group A'
Teichoic Acids
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0378-1097(00)00444-4
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31822
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:05
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Scientific Publication
Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells
193
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel
Marouni, M.J., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel
Perry, R., Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel
Barzilai, A., Pediatric Infectious Diseases Unit, Chaim Sheba Medical Center, Tel-Hashomer Hospital, Tel-Hashomer, Israel
Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells
Lipoteichoic acid (LTA) is thought to play a role in the interactions between Streptococcus pyogenes and host cells. We have examined the effect of exogenous LTA on the adherence and entry of S. pyogenes JRS4 strain into HEp-2 epithelial cells. LTA markedly inhibited bacterial entry in a concentration-dependent manner, up to 250 μg ml-1. In contrast, LTA had only a slight inhibitory effect on adherence. LTA also inhibited the entry but not adherence of Salmonella typhimurium strain into HEp-2 cells. Binding experiments showed a dose-dependent binding of LTA to cells up to 10 μg ml-1. Confocal laser microscopy imaging and analysis revealed that LTA was internalized by the epithelial cells and colocalized with F-actin. These results might imply that, following binding, exogenous LTA enters HEp-2 cells and exerts a cytotoxic effect that interferes with bacterial internalization. A possible target for LTA activity might be the actin cytoskeleton, which is known to be essential for bacterial uptake. Copyright (C) 2000 Federation of European Microbiological Societies.
Scientific Publication
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