אסיף מאגר המחקר החקלאי

Identification of recombinant phages containing sequences from different rat myosin heavy chain genes

Year:

1980

Source of publication :

Authors :

Volume :

8

Co-Authors:

Nudel, U., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Katcoff, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Carmon, Y., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Zevin-Sonkin, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Levi, Z., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Shaul, Y., Departments of Virology, Weizmann Institute of Science, Rehovot, Israel
Shani, M., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Yaffe, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel

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Abstract:

The construction and identification of a recombinant plasmid containing a cDNA Insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated EcoR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes. © 1980 IRL Press Limited.

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