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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of recombinant phages containing sequences from different rat myosin heavy chain genes
Year:
1980
Source of publication :
Nucleic Acids Research
Authors :
שני, משה
;
.
Volume :
8
Co-Authors:
Nudel, U., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Katcoff, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Carmon, Y., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Zevin-Sonkin, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Levi, Z., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Shaul, Y., Departments of Virology, Weizmann Institute of Science, Rehovot, Israel
Shani, M., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Yaffe, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
2133
To page:
2146
(
Total pages:
14
)
Abstract:
The construction and identification of a recombinant plasmid containing a cDNA Insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated EcoR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes. © 1980 IRL Press Limited.
Note:
Related Files :
animal experiment
Animals
gene amplification
Genes
Heredity
myosin
Nucleic Acid Heteroduplexes
RNA, Messenger
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/nar/8.10.2133
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31862
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:05
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Scientific Publication
Identification of recombinant phages containing sequences from different rat myosin heavy chain genes
8
Nudel, U., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Katcoff, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Carmon, Y., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Zevin-Sonkin, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Levi, Z., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Shaul, Y., Departments of Virology, Weizmann Institute of Science, Rehovot, Israel
Shani, M., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Yaffe, D., Departments of Cell Biology, Weizmann Institute of Science, Rehovot, Israel
Identification of recombinant phages containing sequences from different rat myosin heavy chain genes
The construction and identification of a recombinant plasmid containing a cDNA Insert which hybridizes specifically to myosin heavy chain mRNA is described. The plasmid was used as a probe to screen a rat genomic library for recombinant phages containing myosin heavy chain sequences. Six clones with approximately 15 k bp inserts each were isolated. Digestion with several restriction enzymes and hybridization of the fractionated DNA with the plasmid probe showed that the clones contained 3 different DNA inserts. Electron microscopy of a heteroduplex made by hybridization of DNA from two clones confirmed that the inserts originated in different genes. Hybridization of size-fractionated EcoR1 digested rat spleen DNA with the cloned probe suggested the existence of at least 5 myosin heavy chain genes. © 1980 IRL Press Limited.
Scientific Publication
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