חיפוש מתקדם
Plant Pathology Journal
Rosner, A., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
A method based on RNA-transcript conformation polymorphism (TCP) was tested for detection of two PVY isolates in a mixed infection. Differences in electrophoretic mobility of RNA transcripts copied from PCR products of each virus isolate enabled the distinction between the two virus isolates in a mixed infection. The identities of the RNA transcripts and hence of the infecting virus isolates were determined by annealing to reference oligonucleotides containing unique strain-specific sequences visualized by retardation of transcript mobility in gel. The ratio at which both virus isolates could be detected was as low as 1:10. The suitability of this procedure for the study of mixed virus infections is discussed.
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הספר "אוצר וולקני"
אודות
תנאי שימוש
The application of RNA transcript conformation polymorphism in resolving mixed infection of PVY isolates
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Rosner, A., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
The application of RNA transcript conformation polymorphism in resolving mixed infection of PVY isolates
A method based on RNA-transcript conformation polymorphism (TCP) was tested for detection of two PVY isolates in a mixed infection. Differences in electrophoretic mobility of RNA transcripts copied from PCR products of each virus isolate enabled the distinction between the two virus isolates in a mixed infection. The identities of the RNA transcripts and hence of the infecting virus isolates were determined by annealing to reference oligonucleotides containing unique strain-specific sequences visualized by retardation of transcript mobility in gel. The ratio at which both virus isolates could be detected was as low as 1:10. The suitability of this procedure for the study of mixed virus infections is discussed.
Scientific Publication
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