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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Distribution of Salmonella typhimurium in romaine lettuce leaves
Year:
2011
Authors :
בלאוסוב, אדוארד
;
.
סלע, שלמה
;
.
פינטו, רבקה
;
.
קרופיצקי, יוליה
;
.
Volume :
28
Co-Authors:
Kroupitski, Y., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Belausov, E., Confocal Microscopy Unit, Agricultural Research Organization (ARO), The Volcani Center, Beth-Dagan, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Facilitators :
From page:
990
To page:
997
(
Total pages:
8
)
Abstract:
Leafy greens are occasionally involved in outbreaks of enteric pathogens. In order to control the plant contamination it is necessary to understand the factors that influence enteric pathogen-plant interactions. Attachment of Salmonella enterica serovar typhimurium to lettuce leaves has been demonstrated before; however, only limited information is available regarding the localization and distribution of immigrant Salmonella on the leaf surface. To extend our knowledge regarding initial pathogen-leaf interactions, the distribution of green-fluorescent protein-labeled Salmonella typhimurium on artificially contaminated romaine lettuce leaves was analyzed. We demonstrate that attachment of Salmonella to different leaf regions is highly variable; yet a higher attachment level was observed on leaf regions localized close to the petiole (7.7 log CFU g-1) compared to surfaces at the far-end region of the leaf blade (6.2 log CFU g-1). Attachment to surfaces located at a central leaf region demonstrated intermediate attachment level (7.0 log CFU g-1). Salmonella displayed higher affinity toward the abaxial side compared to the adaxial side of the same leaf region. Rarely, Salmonella cells were also visualized underneath stomata within the parenchymal tissue, supporting the notion that this pathogen can also internalize romaine lettuce leaves. Comparison of attachment to leaves of different ages showed that Salmonella displayed higher affinity to older compared to younger leaves (1.5 log). Scanning electron microscopy revealed a more complex topography on the surface of older leaves, as well as on the abaxial side of the examined leaf tissue supporting the notion that a higher attachment level might be correlated with a more composite leaf landscape. Our findings indicate that initial attachment of Salmonella to romaine lettuce leaf depends on multiple plant factors pertaining to the specific localization on the leaf tissue and to the developmental stage of the leaf. © 2011 Elsevier Ltd.
Note:
Related Files :
attachment
Food Contamination
Lactuca
Lettuce
Microbiology
Salmonella
Salmonella enterica subsp. enterica serovar Typhimurium
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.fm.2011.01.007
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31883
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:06
You may also be interested in
Scientific Publication
Distribution of Salmonella typhimurium in romaine lettuce leaves
28
Kroupitski, Y., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Pinto, R., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Belausov, E., Confocal Microscopy Unit, Agricultural Research Organization (ARO), The Volcani Center, Beth-Dagan, Israel
Sela, S., Microbial Food-Safety Research Unit, Department of Food Quality and Safety, Institute for Postharvest Technology and Food Sciences, Agricultural Research Organization (ARO), The Volcani Center, POB 6, Beth-Dagan 50250, Israel
Distribution of Salmonella typhimurium in romaine lettuce leaves
Leafy greens are occasionally involved in outbreaks of enteric pathogens. In order to control the plant contamination it is necessary to understand the factors that influence enteric pathogen-plant interactions. Attachment of Salmonella enterica serovar typhimurium to lettuce leaves has been demonstrated before; however, only limited information is available regarding the localization and distribution of immigrant Salmonella on the leaf surface. To extend our knowledge regarding initial pathogen-leaf interactions, the distribution of green-fluorescent protein-labeled Salmonella typhimurium on artificially contaminated romaine lettuce leaves was analyzed. We demonstrate that attachment of Salmonella to different leaf regions is highly variable; yet a higher attachment level was observed on leaf regions localized close to the petiole (7.7 log CFU g-1) compared to surfaces at the far-end region of the leaf blade (6.2 log CFU g-1). Attachment to surfaces located at a central leaf region demonstrated intermediate attachment level (7.0 log CFU g-1). Salmonella displayed higher affinity toward the abaxial side compared to the adaxial side of the same leaf region. Rarely, Salmonella cells were also visualized underneath stomata within the parenchymal tissue, supporting the notion that this pathogen can also internalize romaine lettuce leaves. Comparison of attachment to leaves of different ages showed that Salmonella displayed higher affinity to older compared to younger leaves (1.5 log). Scanning electron microscopy revealed a more complex topography on the surface of older leaves, as well as on the abaxial side of the examined leaf tissue supporting the notion that a higher attachment level might be correlated with a more composite leaf landscape. Our findings indicate that initial attachment of Salmonella to romaine lettuce leaf depends on multiple plant factors pertaining to the specific localization on the leaf tissue and to the developmental stage of the leaf. © 2011 Elsevier Ltd.
Scientific Publication
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