Co-Authors:
Spiegel-Roy, P., Dept. of Fruit Breeding and Genetics, ARO, Volcani Center, 50 250 Bet Dagan, Israel
Saad, S., Dept. of Fruit Breeding and Genetics, ARO, Volcani Center, 50 250 Bet Dagan, Israel
Abstract:
Stable salt tolerance lines have been selected from Citrus sinensis nucellar callus subjected to solid media containing 0.085 M NaCl for ten passages. Na+, K+, and Cl- content in callus, embryos, plantlets and greenhouse-grown plants has been determined in selected lines and in an unselected control line. Salt tolerant line R4 had a much higher Cl-, Na+ and K+ content in callus than the control. No embryos could be regenerated from R4. Regenerating lines R13 and R14 had a significantly lower Cl-, and in some cases, lower Na+ content in callus than the control. Embryogenesis in these lines was enhanced by addition of glycerol and antigibberellins to the solid medium. A relatively lower Cl- content was also maintained in embryos as well as roots and leaves of plantlets of R14. Shoot proliferation promoted by cytokinins enabled a comparison of plant response of selected line R14 with control. In greenhouse experiments irrigated with saline water (up to 2000-2500 mg Cl/l) R14 showed increased plant height and higher plant survival compared to the wild line.
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