Sharon, E., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Bar-Eyal, M., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Chet, I., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Herrera-Estrella, A., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Kleifeld, O., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Spiegel, Y., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
The fungal biocontrol agent, Trichoderma harzianum, was evaluated for its potential to control the root-knot nematode Meloidogyne javanica. In greenhouse experiments, root galling was reduced and top fresh weight increased in nematode-infected tomatoes following soil pretreatment with Trichoderma peat-bran preparations. The use of a proteinase Prb1-transformed line (P-2) that contains multiple copies of this gene improved biocontrol activity in the greenhouse experiments compared with the nontransformed wild-type strain (WT). All the Trichoderma strains showed the ability to colonize M. javanica-separated eggs and second-stage juveniles (J2) in sterile in vitro assays, whereas P-2 also penetrated the egg masses. This protease-transformed line presented the same nematicidal and overall proteolytic activity as the WT in in vitro tests in which concentrated soil extracts from Trichoderma-treated soils immobilized the infective J2. However, the J2 immobilization and proteolytic activities of both P-2 and the WT were higher than those obtained with strain T-203. Characterization of the activity of all Trichoderma strains soil extracts on J2 showed that it was heat resistant and restricted to the low-molecular-weight fraction (less than 3 kDa). It is suggested that improved proteolytic activity of the antagonist may be important for the biological control of the nematodes.
Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum
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Sharon, E., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Bar-Eyal, M., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Chet, I., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Herrera-Estrella, A., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Kleifeld, O., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel Spiegel, Y., Department of Nematology, Agricultural Res. Organization (ARO), Volcani Center, P.O.B. 6, Bet-Dagan 50250, Israel
Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum
The fungal biocontrol agent, Trichoderma harzianum, was evaluated for its potential to control the root-knot nematode Meloidogyne javanica. In greenhouse experiments, root galling was reduced and top fresh weight increased in nematode-infected tomatoes following soil pretreatment with Trichoderma peat-bran preparations. The use of a proteinase Prb1-transformed line (P-2) that contains multiple copies of this gene improved biocontrol activity in the greenhouse experiments compared with the nontransformed wild-type strain (WT). All the Trichoderma strains showed the ability to colonize M. javanica-separated eggs and second-stage juveniles (J2) in sterile in vitro assays, whereas P-2 also penetrated the egg masses. This protease-transformed line presented the same nematicidal and overall proteolytic activity as the WT in in vitro tests in which concentrated soil extracts from Trichoderma-treated soils immobilized the infective J2. However, the J2 immobilization and proteolytic activities of both P-2 and the WT were higher than those obtained with strain T-203. Characterization of the activity of all Trichoderma strains soil extracts on J2 showed that it was heat resistant and restricted to the low-molecular-weight fraction (less than 3 kDa). It is suggested that improved proteolytic activity of the antagonist may be important for the biological control of the nematodes.