Regeneration of plantlets and bulblets from explants and callus of Allium aflatunense cultivars and selection from indigenous israeli Allium ampeloprasum

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עברית

מדריך מקוצר למשתמש מדריך מפורט למשתמש

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הספר "אוצר וולקני"

אודות

תנאי שימוש

פותח על ידי קלירמאש פתרונות בע"מ -

Regeneration of plantlets and bulblets from explants and callus of Allium aflatunense cultivars and selection from indigenous israeli Allium ampeloprasum

Year: 

1997

Source of publication :

Acta Horticulturae

Authors :

אבנור, דליה

;

אפגין, לוי

;

ותד, עבד

;

ליליין-קיפניס, חנה

;

ניסים-לוי, עדה

.

Volume :

430

Co-Authors: 

Evenor, D., Dept. of Ornamental Horticulture, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Levi-Nissim, A., Dept. of Ornamental Horticulture, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Afgin, L., Dept. of Ornamental Horticulture, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Lilien-Kipnis, H., Dept. of Ornamental Horticulture, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel
Watad, A.A., Dept. of Ornamental Horticulture, Agricultural Research Organization, P.O. Box 6, Bet Dagan 50250, Israel

From page: 

325

To page: 

330

(

Total pages: 

6

)

Link :

Regeneration of plantlets and bulblets from explants and callus of Allium aflatunense cultivars and selection from indigenous israeli Allium ampeloprasum

Abstract: 

Allium aflatunense is a bulbous plant, grown in gardens and also as cut flowers. A. aflatunense cultivars are propagated asexully from bulbs or bulblets. The two main constraints on growing this plant are a low multiplication rate and the high cost of bulb production. Tissue culture technology enables large scale propagation of plantlets and bulblets. Protocols have been developed for initiating callus and regenerating whole plantlets and bulblets from explants derived from different organs of cvs. 'Lucy Ball'; 'Gladiator' and 'Purple Sensation'. Within a short period of time, hundreds of plantlets and bulblets were obtained from a single bulb. Direct organogenesis of shoots or bulblets was obtained on Murashige and Skoog (MS) media containing NAA and BA. Plantlet regeneration from embryogenic like callus was obtained with different combinations of IAA and Kinetin. Explants derived from young inflorescences gave the highest regeneration rate. For further development small bulblets were subcultured onto media with a high sucrose levels, and were incubated at low temperature. Shoot proliferation in selections made from native populations of A. ampeloprasum was induced from shoot tips cultured on MS medium containing a high ratio of Kinetin to IAA and from young florets cultured on MS media with NAA and BA.

Allium

Allium aflatunense

Allium ampeloprasum

Basal plate

inflorescence

Leaf bases

micropropagation

Shoot tips

More details