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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of Colletotrichum acutatum, the causal species of anthracnose of strawberry and other hosts in Bulgaria
Year:
2009
Source of publication :
Acta Horticulturae
Authors :
מימון, מרסל
;
.
מינץ, דרור
;
.
פרימן, סטנלי
;
.
Volume :
842
Co-Authors:
Maymon, M., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Jelev, Z.J., Agricultural University, 4000 Plovdiv, Bulgaria
Bobev, S.G., Agricultural University, 4000 Plovdiv, Bulgaria
Minz, D., Institute for Soil, Water and Environmental Sciences, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Freeman, S., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Facilitators :
From page:
195
To page:
198
(
Total pages:
4
)
Abstract:
Strawberry anthracnose was first reported in Bulgaria in 2002. Since then, the disease has reached epidemic levels in production fields. More than one-hundred Colletotrichum cultures were isolated from different strawberry cultivars, pepper and tomato fruits, and the weed species Cirsium arvense that were cultivated in close proximity with infected strawberry field crops. The Colletotrichum isolates were identified and characterized by classical morphological criteria and by various molecular methods. Morphological characterization identified all the tested isolates as C. acutatum, which was further confirmed by species-specific polymerase chain reaction (PCR) amplification using the universal ITS4 and CaInt2 primers. No PCR products were amplified using C. gloeosporoides specific primers. Arbitrarilyprimed PCR determined that all the studied isolates were uniform, regardless of origin of plant species, indicating that they belong to an asexually reproducing population. Sequence analysis of the complete internal transcribed spacer (ITS) region confirmed that the populations belonged to the species C. acutatum. Pathogenicity tests using representative isolates caused cross infections on the respective alternative hosts. These findings emphasize that various crops including weeds may serve as a potential inoculum source for C. acutatum.
Note:
Related Files :
Cirsium
Colletotrichum
Fragaria ananassa
host specificity
Percent sequence similarity
Rdna and its sequence analysis
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31953
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:06
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Scientific Publication
Characterization of Colletotrichum acutatum, the causal species of anthracnose of strawberry and other hosts in Bulgaria
842
Maymon, M., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Jelev, Z.J., Agricultural University, 4000 Plovdiv, Bulgaria
Bobev, S.G., Agricultural University, 4000 Plovdiv, Bulgaria
Minz, D., Institute for Soil, Water and Environmental Sciences, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Freeman, S., Dept. of Plant Pathology, ARO, Volcani Research Center, Bet Dagan 50250, Israel
Characterization of Colletotrichum acutatum, the causal species of anthracnose of strawberry and other hosts in Bulgaria
Strawberry anthracnose was first reported in Bulgaria in 2002. Since then, the disease has reached epidemic levels in production fields. More than one-hundred Colletotrichum cultures were isolated from different strawberry cultivars, pepper and tomato fruits, and the weed species Cirsium arvense that were cultivated in close proximity with infected strawberry field crops. The Colletotrichum isolates were identified and characterized by classical morphological criteria and by various molecular methods. Morphological characterization identified all the tested isolates as C. acutatum, which was further confirmed by species-specific polymerase chain reaction (PCR) amplification using the universal ITS4 and CaInt2 primers. No PCR products were amplified using C. gloeosporoides specific primers. Arbitrarilyprimed PCR determined that all the studied isolates were uniform, regardless of origin of plant species, indicating that they belong to an asexually reproducing population. Sequence analysis of the complete internal transcribed spacer (ITS) region confirmed that the populations belonged to the species C. acutatum. Pathogenicity tests using representative isolates caused cross infections on the respective alternative hosts. These findings emphasize that various crops including weeds may serve as a potential inoculum source for C. acutatum.
Scientific Publication
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