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פותח על ידי קלירמאש פתרונות בע"מ -
A nylon filter A-ELISA for detecting viruses in water
Year:
1993
Source of publication :
Water Science and Technology
Authors :
נאסר, אחמד
;
.
Volume :
27
Co-Authors:


Elkana, Y., Division of Environmental Science, The Hebrew University, Jerusalem, Israel
Goldstein, L., Division of Environmental Science, The Hebrew University, Jerusalem, Israel

Facilitators :
From page:
135
To page:
141
(
Total pages:
7
)
Abstract:
This study was designed to develop a modification of A-ELISA performed in microtitre plates. Nylon filters have been utilized successfully as a solid phase for the performance of A-ELISA. The use of nylon filters resulted in lower background than nitro-cellulose and paper filters, indicating their suitability as a solid phase for developing A-ELISA. With enteric viruses, human rotaviruses and MS-2 coliphage, negative results were obtained, suggesting high specificity of the developed technique for poliovirus 1. The sensitivity of the developed A-ELISA has been shown to be at least one order of magnitude greater than ordinary ELISA. A positive result with the nylon A-ELISA can be obtained with samples containing 100-1000 pfu/ml of poliovirus. Up to date methods used for detecting viruses in water are elaborate, time consuming and costly. Applying the nylon A-ELISA may overcome some of these disadvantages.
Note:
Related Files :
Conference paper
Filtration
poliovirus 1
Viruses
waste water management
wastewater treatment
water
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
31979
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:06
You may also be interested in
Scientific Publication
A nylon filter A-ELISA for detecting viruses in water
27


Elkana, Y., Division of Environmental Science, The Hebrew University, Jerusalem, Israel
Goldstein, L., Division of Environmental Science, The Hebrew University, Jerusalem, Israel

A nylon filter A-ELISA for detecting viruses in water
This study was designed to develop a modification of A-ELISA performed in microtitre plates. Nylon filters have been utilized successfully as a solid phase for the performance of A-ELISA. The use of nylon filters resulted in lower background than nitro-cellulose and paper filters, indicating their suitability as a solid phase for developing A-ELISA. With enteric viruses, human rotaviruses and MS-2 coliphage, negative results were obtained, suggesting high specificity of the developed technique for poliovirus 1. The sensitivity of the developed A-ELISA has been shown to be at least one order of magnitude greater than ordinary ELISA. A positive result with the nylon A-ELISA can be obtained with samples containing 100-1000 pfu/ml of poliovirus. Up to date methods used for detecting viruses in water are elaborate, time consuming and costly. Applying the nylon A-ELISA may overcome some of these disadvantages.
Scientific Publication
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