אסיף מאגר המחקר החקלאי

A nylon filter A-ELISA for detecting viruses in water

Year:

1993

Source of publication :

Authors :

Volume :

27

Co-Authors:

Elkana, Y., Division of Environmental Science, The Hebrew University, Jerusalem, Israel
Goldstein, L., Division of Environmental Science, The Hebrew University, Jerusalem, Israel

Facilitators :

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Abstract:

This study was designed to develop a modification of A-ELISA performed in microtitre plates. Nylon filters have been utilized successfully as a solid phase for the performance of A-ELISA. The use of nylon filters resulted in lower background than nitro-cellulose and paper filters, indicating their suitability as a solid phase for developing A-ELISA. With enteric viruses, human rotaviruses and MS-2 coliphage, negative results were obtained, suggesting high specificity of the developed technique for poliovirus 1. The sensitivity of the developed A-ELISA has been shown to be at least one order of magnitude greater than ordinary ELISA. A positive result with the nylon A-ELISA can be obtained with samples containing 100-1000 pfu/ml of poliovirus. Up to date methods used for detecting viruses in water are elaborate, time consuming and costly. Applying the nylon A-ELISA may overcome some of these disadvantages.

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