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פותח על ידי קלירמאש פתרונות בע"מ -
A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance
Year:
2001
Source of publication :
Current Genetics
Authors :
דרובי, סמיר
;
.
Volume :
40
Co-Authors:
Yehuda, H., Department of Molecular Genetics, MIGAL Galilee Technological Center, P.O. Box 90000, Rosh Pina 12100, Israel
Droby, S., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wisniewski, M., Appalachian Fruit Research Station, USDA-ARS, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Goldway, M., Department of Molecular Genetics, MIGAL Galilee Technological Center, P.O. Box 90000, Rosh Pina 12100, Israel
Facilitators :
From page:
282
To page:
287
(
Total pages:
6
)
Abstract:
Lithium acetate transformation and electroporation were applied to the biocontrol yeast, Candida oleophila. The hygromycin B resistance gene, flanked by the phosphoglycerate kinase promoter and terminator of Candida tropicalis, served as the genetic selection marker. The transformation efficiency of electroporation was almost 400 times more efficient than that of the lithium acetate method. While incorporation of DNA, flanked by a sequence endogenous to C. oleophila, transpired apparently by homologous recombination, the integration of DNA (that did not contain C. oleophila DNA) occurred at random. Whereas transformants were observed with a linear segment of the plasmid, none were detected with the undigested plasmid. This system provides both a tool for the molecular analysis of the biocontrol mechanism of C. oleophila and a means of tagging C. oleophila for field studies.
Note:
Related Files :
biological control
biotransformation
DNA
drug resistance
genetic markers
mutation
Plasmid
Yeast
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00294-001-0255-x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32034
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
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Scientific Publication
A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance
40
Yehuda, H., Department of Molecular Genetics, MIGAL Galilee Technological Center, P.O. Box 90000, Rosh Pina 12100, Israel
Droby, S., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Wisniewski, M., Appalachian Fruit Research Station, USDA-ARS, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Goldway, M., Department of Molecular Genetics, MIGAL Galilee Technological Center, P.O. Box 90000, Rosh Pina 12100, Israel
A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance
Lithium acetate transformation and electroporation were applied to the biocontrol yeast, Candida oleophila. The hygromycin B resistance gene, flanked by the phosphoglycerate kinase promoter and terminator of Candida tropicalis, served as the genetic selection marker. The transformation efficiency of electroporation was almost 400 times more efficient than that of the lithium acetate method. While incorporation of DNA, flanked by a sequence endogenous to C. oleophila, transpired apparently by homologous recombination, the integration of DNA (that did not contain C. oleophila DNA) occurred at random. Whereas transformants were observed with a linear segment of the plasmid, none were detected with the undigested plasmid. This system provides both a tool for the molecular analysis of the biocontrol mechanism of C. oleophila and a means of tagging C. oleophila for field studies.
Scientific Publication
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