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פותח על ידי קלירמאש פתרונות בע"מ -
Distribution and diversity of archaea corresponding to the limnological cycle of a hypersaline stratified lake (Solar Lake, Sinai, Egypt)
Year:
2000
Authors :
מינץ, דרור
;
.
סיטרין, אדי
;
.
Volume :
66
Co-Authors:
Cytryn, E., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Minz, D., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Oremland, R.S., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Cohen, Y., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Facilitators :
From page:
3269
To page:
3276
(
Total pages:
8
)
Abstract:
The vertical and seasonal distribution and diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development of stratification and mixing. Archaeal sequences were studied via phylogenetic analysis of 16S rDNA sequences as well as denaturing gradient gel electrophoresis analysis. The 165 clones studied were grouped into four phylogenetically different clusters. Most of the clones isolated from both the aerobic epilimnion and the sulfide-rich hypolimnion were defined as cluster I, belonging to the Halobacteriaceae family. The three additional clusters were all isolated from the anaerobic hypolimnion. Cluster II is phylogenetically located between the genera Methanobacterium and Methanococcus. Clusters III and IV relate to two previously documented groups of uncultured euryarchaeota, remotely related to the genus Thermoplasma. No crenarchaeota were found in the water column of the Solar Lake. The archaeal community in the Solar Lake under both stratified and mixed conditions was dominated by halobacteria in salinities higher than 10%. During stratification, additional clusters, some of which may possibly relate to uncultured halophilic methanogens, were found in the sulfide- and methane-rich hypolimnion.
Note:
Related Files :
DNA, Archaeal
DNA, Ribosomal
Electrophoresis, Polyacrylamide Gel
salinity
sequence analysis
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.66.8.3269-3276.2000
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32043
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
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Scientific Publication
Distribution and diversity of archaea corresponding to the limnological cycle of a hypersaline stratified lake (Solar Lake, Sinai, Egypt)
66
Cytryn, E., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Minz, D., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Oremland, R.S., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Cohen, Y., Div. of Microbial and Molec. Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Distribution and diversity of archaea corresponding to the limnological cycle of a hypersaline stratified lake (Solar Lake, Sinai, Egypt)
The vertical and seasonal distribution and diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development of stratification and mixing. Archaeal sequences were studied via phylogenetic analysis of 16S rDNA sequences as well as denaturing gradient gel electrophoresis analysis. The 165 clones studied were grouped into four phylogenetically different clusters. Most of the clones isolated from both the aerobic epilimnion and the sulfide-rich hypolimnion were defined as cluster I, belonging to the Halobacteriaceae family. The three additional clusters were all isolated from the anaerobic hypolimnion. Cluster II is phylogenetically located between the genera Methanobacterium and Methanococcus. Clusters III and IV relate to two previously documented groups of uncultured euryarchaeota, remotely related to the genus Thermoplasma. No crenarchaeota were found in the water column of the Solar Lake. The archaeal community in the Solar Lake under both stratified and mixed conditions was dominated by halobacteria in salinities higher than 10%. During stratification, additional clusters, some of which may possibly relate to uncultured halophilic methanogens, were found in the sulfide- and methane-rich hypolimnion.
Scientific Publication
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