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פותח על ידי קלירמאש פתרונות בע"מ -
Impact of short-term acidification on nitrification and nitrifying bacterial community dynamics in soilless cultivation media
Year:
2012
Authors :
זילבר, אבנר
;
.
לבקוביץ', אירית
;
.
נגראנו, יעל
;
.
סיטרין, אדי
;
.
Volume :
78
Co-Authors:
Cytryn, E., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Levkovitch, I., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Negreanu, Y., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Dowd, S., MR DNA Molecular Research LP, Shallowater, TX, United States
Frenk, S., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Silber, A., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Facilitators :
From page:
6576
To page:
6582
(
Total pages:
7
)
Abstract:
Soilless medium-based horticulture systems are highly prevalent due to their capacity to optimize growth of high-cash crops. However, these systems are highly dynamic and more sensitive to physiochemical and pH perturbations than traditional soilbased systems, especially during nitrification associated with ammonia-based fertilization. The objective of this study was to assess the impact of nitrification-generated acidification on ammonia oxidation rates and nitrifying bacterial community dynamics in soilless growth media. To achieve this goal, perlite soilless growth medium from a commercial bell pepper greenhouse was incubated with ammonium in bench-scale microcosm experiments. Initial quantitative real-time PCR analysis indicated that betaproteobacterial ammonia oxidizers were significantly more abundant than ammonia-oxidizing archaea, and therefore, research focused on this group. Ammonia oxidation rates were highest between 0 and 9 days, when pH values dropped from 7.4 to 4.9. Pyrosequencing of betaproteobacterial ammonia-oxidizing amoA gene fragments indicated that r-strategist-like Nitrosomonas was the dominant ammonia-oxidizing bacterial genus during this period, seemingly due to the high ammonium concentration and optimal growth conditions in the soilless media. Reduction of pH to levels below 4.8 resulted in a significant decrease in both ammonia oxidation rates and the diversity of ammonia-oxidizing bacteria, with increased relative abundance of the r-strategist-like Nitrosospira. Nitrite oxidizers (Nitrospira and Nitrobacter) were on the whole more abundant and less sensitive to acidification than ammonia oxidizers. This study demonstrates that nitrification and nitrifying bacterial community dynamics in high-N-load intensive soilless growth media may be significantly different from those in in-terra agricultural systems. © 2012, American Society for Microbiology.
Note:
Related Files :
bacteria
fertilization (reproduction)
Genes
Genetics
horticulture
metabolism
oxidation
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.01545-12
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32094
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
You may also be interested in
Scientific Publication
Impact of short-term acidification on nitrification and nitrifying bacterial community dynamics in soilless cultivation media
78
Cytryn, E., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Levkovitch, I., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Negreanu, Y., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Dowd, S., MR DNA Molecular Research LP, Shallowater, TX, United States
Frenk, S., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Silber, A., Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
Impact of short-term acidification on nitrification and nitrifying bacterial community dynamics in soilless cultivation media
Soilless medium-based horticulture systems are highly prevalent due to their capacity to optimize growth of high-cash crops. However, these systems are highly dynamic and more sensitive to physiochemical and pH perturbations than traditional soilbased systems, especially during nitrification associated with ammonia-based fertilization. The objective of this study was to assess the impact of nitrification-generated acidification on ammonia oxidation rates and nitrifying bacterial community dynamics in soilless growth media. To achieve this goal, perlite soilless growth medium from a commercial bell pepper greenhouse was incubated with ammonium in bench-scale microcosm experiments. Initial quantitative real-time PCR analysis indicated that betaproteobacterial ammonia oxidizers were significantly more abundant than ammonia-oxidizing archaea, and therefore, research focused on this group. Ammonia oxidation rates were highest between 0 and 9 days, when pH values dropped from 7.4 to 4.9. Pyrosequencing of betaproteobacterial ammonia-oxidizing amoA gene fragments indicated that r-strategist-like Nitrosomonas was the dominant ammonia-oxidizing bacterial genus during this period, seemingly due to the high ammonium concentration and optimal growth conditions in the soilless media. Reduction of pH to levels below 4.8 resulted in a significant decrease in both ammonia oxidation rates and the diversity of ammonia-oxidizing bacteria, with increased relative abundance of the r-strategist-like Nitrosospira. Nitrite oxidizers (Nitrospira and Nitrobacter) were on the whole more abundant and less sensitive to acidification than ammonia oxidizers. This study demonstrates that nitrification and nitrifying bacterial community dynamics in high-N-load intensive soilless growth media may be significantly different from those in in-terra agricultural systems. © 2012, American Society for Microbiology.
Scientific Publication
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