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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of an epiphytic yeast population of grapefruit capable of suppression of green mold decay caused by Penicillium digitatum
Year:
1999
Source of publication :
biological control (source)
Authors :
דעוס, אבינועם
;
.
דרובי, סמיר
;
.
וייס, בתיה
;
.
כהן, לידיה
;
.
לישצ'ינסקי, ס'
;
.
מנוליס-ששון, שולמית
;
.
Volume :
16
Co-Authors:
Droby, S., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Lischinski, S., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Cohen, L., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Weiss, B., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Daus, A., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Chand-Goyal, T., Environmental Protection Agency, 401 M Street SW, Washington, DC 20460, United States
Eckert, J.W., Department of Plant Pathology, University of California, Riverside, CA 92521, United States
Manulis, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
27
To page:
34
(
Total pages:
8
)
Abstract:
An epiphytic yeast population of grapefruit able to grow under high osmotic conditions and a wide range of temperatures was isolated and characterized for its biocontrol activity against green mold decay caused by Penicillium digitatum. Techniques based on random amplified polymorphic DNA (RAPD) and arbitrary primed polymerase chain reaction (ap-PCR), as well as homologies between sequences of the rDNA internal transcribed spacers (ITS) and 5.8S gene, were used to characterize the composition of the yeast population and to determine the genetic relationships among predominant yeast species. Epiphytic yeasts exhibiting the highest biocontrol activity against P. digitatum on grapefruit were identified as Candida guilliermondii, C. oleophila, C. sake, and Debaryomyces hansenii, while C. guilliermondii was the most predominant species. RAPD and ap-PCR analyses of the osmotolerant yeast population showed two different, major groups. The sequences of the ITS regions and the 5.8S gene of the yeast isolates, previously identified as belonging to different species, were found to be identical.
Note:
Related Files :
biological control
biological pest control
Polymerase Chain Reaction
random amplified polymorphic DNA
yeasts
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/bcon.1999.0725
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32121
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
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Scientific Publication
Characterization of an epiphytic yeast population of grapefruit capable of suppression of green mold decay caused by Penicillium digitatum
16
Droby, S., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Lischinski, S., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Cohen, L., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Weiss, B., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Daus, A., Department of Postharvest Science, ARO, Volcani Center, Bet Dagan, 50250, Israel
Chand-Goyal, T., Environmental Protection Agency, 401 M Street SW, Washington, DC 20460, United States
Eckert, J.W., Department of Plant Pathology, University of California, Riverside, CA 92521, United States
Manulis, S., Department of Plant Pathology, ARO, Volcani Center, Bet Dagan, 50250, Israel
Characterization of an epiphytic yeast population of grapefruit capable of suppression of green mold decay caused by Penicillium digitatum
An epiphytic yeast population of grapefruit able to grow under high osmotic conditions and a wide range of temperatures was isolated and characterized for its biocontrol activity against green mold decay caused by Penicillium digitatum. Techniques based on random amplified polymorphic DNA (RAPD) and arbitrary primed polymerase chain reaction (ap-PCR), as well as homologies between sequences of the rDNA internal transcribed spacers (ITS) and 5.8S gene, were used to characterize the composition of the yeast population and to determine the genetic relationships among predominant yeast species. Epiphytic yeasts exhibiting the highest biocontrol activity against P. digitatum on grapefruit were identified as Candida guilliermondii, C. oleophila, C. sake, and Debaryomyces hansenii, while C. guilliermondii was the most predominant species. RAPD and ap-PCR analyses of the osmotolerant yeast population showed two different, major groups. The sequences of the ITS regions and the 5.8S gene of the yeast isolates, previously identified as belonging to different species, were found to be identical.
Scientific Publication
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