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פותח על ידי קלירמאש פתרונות בע"מ -
Multiple species of defective RNAs in plants infected with citrus tristeza virus
Year:
1995
Source of publication :
Virology
Authors :
אשולין, לילך
;
.
בר-יוסף, משה
;
.
גפני, רון
;
.
מוואסי, מוניר
;
.
מיטקיבסקה, אליזבטה
;
.
Volume :
214
Co-Authors:
Mawass, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Mietkiewska, E., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Hilf, M.E., Horticultural Research Laboratory, USDA-ARS, 2120 Camden Road, Orlando, FL 33803, United States
Ashoulin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Karasev, A.V., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Gafny, R., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Lee, R.F., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Garnsey, S.M., Horticultural Research Laboratory, USDA-ARS, 2120 Camden Road, Orlando, FL 33803, United States
Dawson, W.O., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Bar-Joseph, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
264
To page:
268
(
Total pages:
5
)
Abstract:
Alemow (Citrus macrophylla) and sweet orange (C. sinensis) plants infected, respectively, with several lsraeli and Florida isolates of the citrus tristeza virus (CTV) were found to contain multiple species of RNA molecules with features similar to defective-interfering RNAs. Northern blot hybridizations of dsRNAs extracted from serial passages of the Israeli VT isolate (CTV-VT) and from different plants infected with a single source of inoculum showed considerable variation both in the presence and in the relative abundance of the defective RNA (D-RNA) bands. The D-RNA molecules were found to be encapsidated in the CTV particles. Sequence analysis of two VT D-RNA molecules of 2.7 and 4.5 kb revealed that they were composed of two regions corresponding to 1818 and 4036 nucleotides from the 5' and 938 and 442 nucleotides from the 3' termini of the CTV-VT genomic RNA, respectively. A short (ca. 0.8 kb) nonencapsidated single-stranded positive-sense RNA species was also found in infected plants. This ssRNA, which copurified with dsRNAs, was shown by hybridization to encompass the 5'-terminal part of the CTV genome and might have an extensive secondary structure.
Note:
Related Files :
Agriculture
Base Sequence
Citrus tristeza virus
Plant Diseases
RNA
RNA viruses
sweet orange
virus infection
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/viro.1995.9930
Article number:
3000
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32128
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
Scientific Publication
Multiple species of defective RNAs in plants infected with citrus tristeza virus
214
Mawass, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Mietkiewska, E., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Hilf, M.E., Horticultural Research Laboratory, USDA-ARS, 2120 Camden Road, Orlando, FL 33803, United States
Ashoulin, L., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Karasev, A.V., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Gafny, R., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Lee, R.F., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Garnsey, S.M., Horticultural Research Laboratory, USDA-ARS, 2120 Camden Road, Orlando, FL 33803, United States
Dawson, W.O., Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, United States
Bar-Joseph, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Multiple species of defective RNAs in plants infected with citrus tristeza virus
Alemow (Citrus macrophylla) and sweet orange (C. sinensis) plants infected, respectively, with several lsraeli and Florida isolates of the citrus tristeza virus (CTV) were found to contain multiple species of RNA molecules with features similar to defective-interfering RNAs. Northern blot hybridizations of dsRNAs extracted from serial passages of the Israeli VT isolate (CTV-VT) and from different plants infected with a single source of inoculum showed considerable variation both in the presence and in the relative abundance of the defective RNA (D-RNA) bands. The D-RNA molecules were found to be encapsidated in the CTV particles. Sequence analysis of two VT D-RNA molecules of 2.7 and 4.5 kb revealed that they were composed of two regions corresponding to 1818 and 4036 nucleotides from the 5' and 938 and 442 nucleotides from the 3' termini of the CTV-VT genomic RNA, respectively. A short (ca. 0.8 kb) nonencapsidated single-stranded positive-sense RNA species was also found in infected plants. This ssRNA, which copurified with dsRNAs, was shown by hybridization to encompass the 5'-terminal part of the CTV genome and might have an extensive secondary structure.
Scientific Publication
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