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Low oxygen and 1-MCP pretreatments delay superficial scald development by reducing reactive oxygen species (ROS) accumulation in stored 'Granny Smith' apples
Year:
2011
Source of publication :
Postharvest Biology and Technology
Authors :
בלאוסוב, אדוארד
;
.
סבן-אמין, רויטל
;
.
פייגנברג, אולג
;
.
פסיס, עדנה
;
.
Volume :
62
Co-Authors:
Sabban-Amin, R., Dept. of Postharvest Science of Fresh Produce, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Feygenberg, O., Dept. of Postharvest Science of Fresh Produce, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Belausov, E., Dept. of Plant Science, The Volcani Center, Israel
Pesis, E., Dept. of Postharvest Science of Fresh Produce, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
295
To page:
304
(
Total pages:
10
)
Abstract:
'Granny Smith' apples are highly susceptible to superficial scald, a symptom of chilling injury. For many crops, low temperature storage results in oxidative stress and chilling injury, caused by increased production of superoxide anions which in turn leads to the generation of other dangerous reactive oxygen species (ROS). Application, prior to cold storage, of low oxygen (LO2, <0.5%) atmospheres, ethanol (<2% vapour) or 1-methylcyclopropene (1-MCP, 0.5μLL-1) at 20°C, was effective in reducing superficial scald in fruit following 24 weeks of cold storage. ROS levels were measured by confocal laser-scanning microscopy of apple peel treated with the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate. In control fruit, ROS levels increased during cold storage and shelf-life and were very high after only 8 weeks, whereas in 1-MCP-, ethanol- and LO2-treated fruit, ROS levels remained low throughout storage. Gene-expression levels of ROS-scavenging enzymes were induced by the various pretreatments: catalase (MdCAT) was induced by LO2 treatment, whereas Mn superoxide dismutase (MdMnSOD) was induced by 1-MCP treatment. Polyphenol oxidase (MdPPO) gene expression levels were associated with scald symptom development and were highest in control fruit. Ethylene levels and expression of ethylene biosynthesis genes were correlated with α-farnesene levels and <alpha>-farnesene synthase (MdAFS) gene expression in the variously treated fruit. Accumulation of the α-farnesene oxidation product, 6-methyl-5-hepten-2-one (MHO), was highest in control fruit after 8 weeks, in accordance with ROS accumulation. The LO2 pretreatment mechanism might involve production of anaerobic metabolites, causing a delay in ethylene and α-farnesene biosynthesis and oxidation; this is different from the mechansism of action of 1-MCP, even though both consequently reduce ROS accumulation and scald symptoms. © 2011 Elsevier B.V.
Note:
Related Files :
ethanol
ethylene
Fluorescence
Malus x domestica
Malus×domestica
MHO
α-Farnesene
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.postharvbio.2011.06.016
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32132
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
You may also be interested in
Scientific Publication
Low oxygen and 1-MCP pretreatments delay superficial scald development by reducing reactive oxygen species (ROS) accumulation in stored 'Granny Smith' apples
62
Sabban-Amin, R., Dept. of Postharvest Science of Fresh Produce, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Feygenberg, O., Dept. of Postharvest Science of Fresh Produce, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Belausov, E., Dept. of Plant Science, The Volcani Center, Israel
Pesis, E., Dept. of Postharvest Science of Fresh Produce, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Low oxygen and 1-MCP pretreatments delay superficial scald development by reducing reactive oxygen species (ROS) accumulation in stored 'Granny Smith' apples
'Granny Smith' apples are highly susceptible to superficial scald, a symptom of chilling injury. For many crops, low temperature storage results in oxidative stress and chilling injury, caused by increased production of superoxide anions which in turn leads to the generation of other dangerous reactive oxygen species (ROS). Application, prior to cold storage, of low oxygen (LO2, <0.5%) atmospheres, ethanol (<2% vapour) or 1-methylcyclopropene (1-MCP, 0.5μLL-1) at 20°C, was effective in reducing superficial scald in fruit following 24 weeks of cold storage. ROS levels were measured by confocal laser-scanning microscopy of apple peel treated with the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate. In control fruit, ROS levels increased during cold storage and shelf-life and were very high after only 8 weeks, whereas in 1-MCP-, ethanol- and LO2-treated fruit, ROS levels remained low throughout storage. Gene-expression levels of ROS-scavenging enzymes were induced by the various pretreatments: catalase (MdCAT) was induced by LO2 treatment, whereas Mn superoxide dismutase (MdMnSOD) was induced by 1-MCP treatment. Polyphenol oxidase (MdPPO) gene expression levels were associated with scald symptom development and were highest in control fruit. Ethylene levels and expression of ethylene biosynthesis genes were correlated with α-farnesene levels and <alpha>-farnesene synthase (MdAFS) gene expression in the variously treated fruit. Accumulation of the α-farnesene oxidation product, 6-methyl-5-hepten-2-one (MHO), was highest in control fruit after 8 weeks, in accordance with ROS accumulation. The LO2 pretreatment mechanism might involve production of anaerobic metabolites, causing a delay in ethylene and α-farnesene biosynthesis and oxidation; this is different from the mechansism of action of 1-MCP, even though both consequently reduce ROS accumulation and scald symptoms. © 2011 Elsevier B.V.
Scientific Publication
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