Co-Authors:
Shemesh, M., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Steinberg, D., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem 91120, Israel
Abstract:
The extracellular enzyme fructosyltransferase (FTF) is considered to be a significant virulence factor in the dental biofilm. We have developed a method using surface plasmon resonance to detect the activity of immobilized FTF in situ. This real time technique provides a sensitive direct assay for characterizing functional properties of immobilized enzymes such as FTF. © 2005 Elsevier B.V. All rights reserved.