Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides

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Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides

Year: 

1997

Source of publication :

Theoretical and Applied Genetics

Authors :

גרמה, אדריאנה

.

Volume :

Co-Authors: 

Sun, G.L., Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel, Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, 611830, Sichuan, China
Fahima, T., Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel
Korol, A.B., Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel
Turpeinen, T., Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel, MTT Agricultural Research Center, Institute of Crop and Soil Science, SF-31600, Jokioinen, Finland
Grama, A., Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Ronin, Y.I., Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel
Nevo, E., Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel

From page: 

622

To page: 

628

(

Total pages: 

7

)

Link :

Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides

Abstract: 

The Yr15 gene of wheat confers resistance to the stripe rust pathogen Puccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic RAPD bands between the susceptible and the resistant lines. The genetic linkage of the polymorphic markers was tested on segregating F2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generated by the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum-likelihood technique it was found that the order OPB131420 Yr15-Nor1 on chromosome lB appeared to be no less than 1000-times more probable than the closest alternative. The map distances between OPB131420-Yr15-NorI are 27.1 cM and 11.0 cM for the first and second intervals, respectively. The application of marker-assisted selection for the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.