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פותח על ידי קלירמאש פתרונות בע"מ -
Differentiation among isolates of prunus necrotic ringspot virus by transcript conformation polymorphism
Year:
1998
Source of publication :
Journal of Virological Methods
Authors :
מסלנין, לודמילה
;
.
רוזנר, אריה
;
.
שפיגל, שרה
;
.
Volume :
74
Co-Authors:
Rosner, A., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
109
To page:
115
(
Total pages:
7
)
Abstract:
A method based on differences in electrophoretic mobility of RNA transcripts made from polymerase chain reaction (PCR) products was used for differentiation among virus isolates. A T7 RNA polymerase promoter was attached to amplified prunus necrotic ringspot virus (PNRSV) sequences by PCR. The PCR products then served as a template for transcription. Single-stranded transcripts originated from different PNRSV isolates varied in electrophoretic mobility in polyacrylamide gels, presumably because of transcript conformation polymorphism (TCP). This procedure was applied for the differentiation of PNRSV isolates. Copyright (C) 1998 Elsevier Science B.V.
Note:
Related Files :
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Strain differentiation
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0166-0934(98)00076-7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32170
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:07
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Scientific Publication
Differentiation among isolates of prunus necrotic ringspot virus by transcript conformation polymorphism
74
Rosner, A., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Differentiation among isolates of prunus necrotic ringspot virus by transcript conformation polymorphism
A method based on differences in electrophoretic mobility of RNA transcripts made from polymerase chain reaction (PCR) products was used for differentiation among virus isolates. A T7 RNA polymerase promoter was attached to amplified prunus necrotic ringspot virus (PNRSV) sequences by PCR. The PCR products then served as a template for transcription. Single-stranded transcripts originated from different PNRSV isolates varied in electrophoretic mobility in polyacrylamide gels, presumably because of transcript conformation polymorphism (TCP). This procedure was applied for the differentiation of PNRSV isolates. Copyright (C) 1998 Elsevier Science B.V.
Scientific Publication
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