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פותח על ידי קלירמאש פתרונות בע"מ -
Linkage and physical mapping of sex region on LG23 of nile tilapia (oreochromis niloticus)
Year:
2012
Source of publication :
G3: Genes, Genomes, Genetics
Authors :
אשל, אורלי
;
.
ולר, יהודה
;
.
חולתא, גדעון
;
.
רון, מיכה
;
.
שיראק, אנדרי
;
.
Volume :
2
Co-Authors:
Eshel, O., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel, Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Weller, J.I., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Facilitators :
From page:
35
To page:
42
(
Total pages:
8
)
Abstract:
Evidence supports that sex determination (SD) in tilapia is controlled by major genetic factors that may interact with minor genetic as well as environmental factors, thus implying that SD should be analyzed as a quantitative trait. Quantitative trait loci (QTL) for SD in Oreochromis niloticus were previously detected on linkage groups (LG) 1 and 23. Twenty-one short single repeats (SSR) of >12 TGs and one single nucleotide polymorphism were identified using the unpublished tilapia genome sequence on LG23. All markers showed two segregating alleles in a mapping family that was obtained by a cross between O. niloticus male (XY) and sex-reversed female (δXY) yielding 29 females (XX) and 61 males (XY and YY). Interval mapping analysis mapped the QTL peak between SSR markers ARO172 and ARO177 with a maximum F value of 78.7 (P <7.6× 10-14). Twelve adjacent markers found in this region were homozygous in females and either homozygous for the alternative allele or heterozygous in males. This segment was defined as the sex region (SR). The SR encompasses 1.5 Mbp on a single tilapia scaffold (no. 101) harboring 51 annotated genes. Among 10 candidate genes for SD that were tested for gene expression, anti-Müllerian hormone (Amh), which is located in the center of the SR, showed the highest overexpression in male vs. female embryos at 3 to 7 days postfertilization. © 2012 Eshel et al.
Note:
Related Files :
Linkage mapping
microsatellite markers
Oreochromis niloticus
Physical mapping
Sex region
עוד תגיות
תוכן קשור
More details
DOI :
10.1534/g3.111.001545
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32177
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:08
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Scientific Publication
Linkage and physical mapping of sex region on LG23 of nile tilapia (oreochromis niloticus)
2
Eshel, O., Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel, Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Weller, J.I., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, Bet Dagan 50250, Israel
Linkage and physical mapping of sex region on LG23 of nile tilapia (oreochromis niloticus)
Evidence supports that sex determination (SD) in tilapia is controlled by major genetic factors that may interact with minor genetic as well as environmental factors, thus implying that SD should be analyzed as a quantitative trait. Quantitative trait loci (QTL) for SD in Oreochromis niloticus were previously detected on linkage groups (LG) 1 and 23. Twenty-one short single repeats (SSR) of >12 TGs and one single nucleotide polymorphism were identified using the unpublished tilapia genome sequence on LG23. All markers showed two segregating alleles in a mapping family that was obtained by a cross between O. niloticus male (XY) and sex-reversed female (δXY) yielding 29 females (XX) and 61 males (XY and YY). Interval mapping analysis mapped the QTL peak between SSR markers ARO172 and ARO177 with a maximum F value of 78.7 (P <7.6× 10-14). Twelve adjacent markers found in this region were homozygous in females and either homozygous for the alternative allele or heterozygous in males. This segment was defined as the sex region (SR). The SR encompasses 1.5 Mbp on a single tilapia scaffold (no. 101) harboring 51 annotated genes. Among 10 candidate genes for SD that were tested for gene expression, anti-Müllerian hormone (Amh), which is located in the center of the SR, showed the highest overexpression in male vs. female embryos at 3 to 7 days postfertilization. © 2012 Eshel et al.
Scientific Publication
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