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פותח על ידי קלירמאש פתרונות בע"מ -
New trends in gamete's cryopreservation
Year:
2002
Authors :
גסיטוע, חיים
;
.
דקל, יצחק
;
.
שלום, יבין
;
.
Volume :
187
Co-Authors:
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Yavin, S., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Zeron, Y., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Natan, D., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Dekel, I., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Gacitua, H., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
77
To page:
81
(
Total pages:
5
)
Abstract:
We developed new techniques to improve freezing and vitrification of sperm, oocytes and embryos. Our novel freezing technology is based on 'Multi-Thermal-Gradient' (MTG) freezing that is used for sperm. The freezing apparatus has the ability to control ice crystals propagation by changing thermal gradient or the liquid-ice interface velocity which optimizes ice crystals morphology during freezing of cells and tissue. Using this apparatus we were able to freeze bull, stallion, boar, ram, fowl and human sperm with normal post-thaw motility/pre-freezing motility of 70-100%. The vitrification method includes the cooling of nanoliter sample (the 'Minimum Drop Size' technique) in 'super-cooled' liquid nitrogen (-210°C), which maximized cooling rate to the highest physically possible (24-130 000°C/min). Using this method we achieved very high survival of bovine oocytes and embryos. Vitrification of oocytes at the MII stage resulted with cleavage and blastocyst rate of 50 and 20%, respectively. The vitrification of in-vitro production (IVP) of bovine embryos allowed the production of a healthy calf after embryo-transfer carrying the name 'Zegugit' (in Hebrew: made from glass). © 2002 Elsevier Science Ireland Ltd.
Note:
Related Files :
Animal
Animals
animal tissue
cattle
Conference paper
freezing
Germ Cells
Male
Review
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0303-7207(01)00700-6
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32197
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:08
Scientific Publication
New trends in gamete's cryopreservation
187
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Yavin, S., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Zeron, Y., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Natan, D., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Dekel, I., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Gacitua, H., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
New trends in gamete's cryopreservation
We developed new techniques to improve freezing and vitrification of sperm, oocytes and embryos. Our novel freezing technology is based on 'Multi-Thermal-Gradient' (MTG) freezing that is used for sperm. The freezing apparatus has the ability to control ice crystals propagation by changing thermal gradient or the liquid-ice interface velocity which optimizes ice crystals morphology during freezing of cells and tissue. Using this apparatus we were able to freeze bull, stallion, boar, ram, fowl and human sperm with normal post-thaw motility/pre-freezing motility of 70-100%. The vitrification method includes the cooling of nanoliter sample (the 'Minimum Drop Size' technique) in 'super-cooled' liquid nitrogen (-210°C), which maximized cooling rate to the highest physically possible (24-130 000°C/min). Using this method we achieved very high survival of bovine oocytes and embryos. Vitrification of oocytes at the MII stage resulted with cleavage and blastocyst rate of 50 and 20%, respectively. The vitrification of in-vitro production (IVP) of bovine embryos allowed the production of a healthy calf after embryo-transfer carrying the name 'Zegugit' (in Hebrew: made from glass). © 2002 Elsevier Science Ireland Ltd.
Scientific Publication
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