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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of a missense mutation in the bovine ABCG2 gene with a major effect on the QTL on chromosome 6 affecting milk yield and composition in Holstein cattle
Year:
2005
Source of publication :
Genome Research
Authors :
ולר, יהודה
;
.
כהן-צינדר, מירי
;
.
סרוסי, אייל
;
.
רון, מיכה
;
.
שני, משה
;
.
Volume :
15
Co-Authors:
Cohen-Zinder, M., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Seroussi, E., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Larkin, D.M., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Loor, J.J., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Everts-Van Der Wind, A., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Lee, J.-H., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Drackley, J.K., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Band, M.R., W.M. Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL 61801, United States
Hernandez, A.G., W.M. Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL 61801, United States
Shani, M., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Lewin, H.A., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Weller, J.I., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Ron, M., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Facilitators :
From page:
936
To page:
944
(
Total pages:
9
)
Abstract:
We previously localized a quantitative trait locus (QTL) on chromosome 6 affecting milk fat and protein concentration to a 4-cM confidence interval, centered on the microsatellite BM143. We characterized the genes and sequence variation in this region and identified common haplotypes spanning five polymorphic sites in the genes IBSP, SPP1, PKD2, and ABCG2 for two sires heterozygous for this QTL. Expression of SPP1 and ABCG2 in the bovine mammary gland increased from parturition through lactation. SPP1 and all the coding exons of ABCG2 and PKD2 were sequenced for these two sires. The single nucleotide change capable of encoding a substitution of tyrosine-581 to serine (Y581S) in the ABCG2 transporter was the only polymorphism corresponding to the segregation status of all 3 heterozygous and 15 homozygous sires for the QTL in the Israeli and U.S. Holstein populations. The allele substitution fixed effects on the genetic evaluations of 335 Israeli sires were -341 kg milk, +0.16% fat, and +0.13% protein (F-value = 200). No other polymorphism gave significant effect for fat and protein concentration in models that also included Y581S. The allele substitution effects on the genetic evaluations of 670 cows, daughters of two heterozygous sires, were -226 kg milk, 0.09% fat, and 0.08% protein (F-value = 394), with partial dominance towards the 581S homozygotes. We therefore propose that Y581S in ABCG2 is the causative site for this QTL. ©2005 by Cold Spring Harbor Laboratory Press.
Note:
Related Files :
Animals
cattle
Female
gene expression
lactation
milk
milk yield
עוד תגיות
תוכן קשור
More details
DOI :
10.1101/gr.3806705
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32199
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:08
Scientific Publication
Identification of a missense mutation in the bovine ABCG2 gene with a major effect on the QTL on chromosome 6 affecting milk yield and composition in Holstein cattle
15
Cohen-Zinder, M., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Seroussi, E., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Larkin, D.M., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Loor, J.J., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Everts-Van Der Wind, A., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Lee, J.-H., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Drackley, J.K., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Band, M.R., W.M. Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL 61801, United States
Hernandez, A.G., W.M. Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL 61801, United States
Shani, M., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Lewin, H.A., Department of Animal Sciences, University of Illinois, Urbana, IL 61801, United States
Weller, J.I., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Ron, M., Institute of Animal Sciences, Agricultural Research Organization, Bet-Dagan 50250, Israel
Identification of a missense mutation in the bovine ABCG2 gene with a major effect on the QTL on chromosome 6 affecting milk yield and composition in Holstein cattle
We previously localized a quantitative trait locus (QTL) on chromosome 6 affecting milk fat and protein concentration to a 4-cM confidence interval, centered on the microsatellite BM143. We characterized the genes and sequence variation in this region and identified common haplotypes spanning five polymorphic sites in the genes IBSP, SPP1, PKD2, and ABCG2 for two sires heterozygous for this QTL. Expression of SPP1 and ABCG2 in the bovine mammary gland increased from parturition through lactation. SPP1 and all the coding exons of ABCG2 and PKD2 were sequenced for these two sires. The single nucleotide change capable of encoding a substitution of tyrosine-581 to serine (Y581S) in the ABCG2 transporter was the only polymorphism corresponding to the segregation status of all 3 heterozygous and 15 homozygous sires for the QTL in the Israeli and U.S. Holstein populations. The allele substitution fixed effects on the genetic evaluations of 335 Israeli sires were -341 kg milk, +0.16% fat, and +0.13% protein (F-value = 200). No other polymorphism gave significant effect for fat and protein concentration in models that also included Y581S. The allele substitution effects on the genetic evaluations of 670 cows, daughters of two heterozygous sires, were -226 kg milk, 0.09% fat, and 0.08% protein (F-value = 394), with partial dominance towards the 581S homozygotes. We therefore propose that Y581S in ABCG2 is the causative site for this QTL. ©2005 by Cold Spring Harbor Laboratory Press.
Scientific Publication
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