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פותח על ידי קלירמאש פתרונות בע"מ -
ATP synthesis in cell-free extracts of peanut (Arachis hypogaea L.) seeds
Year:
1982
Source of publication :
Journal of Experimental Botany
Authors :
פרל, מאיר
;
.
Volume :
33
Co-Authors:
Perl, M., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
463
To page:
470
(
Total pages:
8
)
Abstract:
Cell-free extracts of peanut (Arachis hypogaea L., cv. Shulamit) seeds, incubated with various substrates, synthesized ATP. Significant synthesis occurred in the presence of AMP + PEP, NADH 2 + PEP and NAD + PEP. When the activities were examined in extracts prepared with 0.3 M mannitol, the rates were 0.6, 0.1 and 0.04 nmol min -1 mg -1 protein, respectively. The activities under such conditions were linear with time up to 90 min incubation at 30 °C. In the presence of PEP + NADH 2 there was a higher specific activity in extracts from non-dormant seeds than from dormant seeds. No such difference was found when PEP + AMP or NAD + PEP was used as the substrate. The temperature dependence of the activity showed a relatively high energy of activation (Ea) for AMP + PEP and a low one if NADH 2 + PEP or NAD + PEP was used as substrate. In buffer extracts of seeds ATP was synthesized in the presence of the above-mentioned substrate combinations but the rate of activity exhibited a lag phase at the early time of incubation, after which higher rates of activities (as compared with mannitol extracts) were obtained. The activities were Co +-dependent, with a K m of about 0.7 mM. In the buffer extracts relatively high activities of adenylate kinase (EC 2.7.4.3 (AK) and pyruvate kinase (EC 2.7.1.50) (PK) were found. AK was stimulated by ethephon (ethylene). This effect is temperature-dependent and occurs in both directions: in the presence of ADP (→ATP + AMP) as well as if ATP + AMP is used as substrate to synthesize ADP. PK is Co +-dependent, and unaffected by ethephon. Both activities were stimulated by malonate. © 1982 Oxford University Press.
Note:
Related Files :
Adenylate Kinase
Arachis hypogaea
ATP synthesis
peanut
pyruvate kinase
Seed
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/jxb/33.3.463
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32205
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:08
Scientific Publication
ATP synthesis in cell-free extracts of peanut (Arachis hypogaea L.) seeds
33
Perl, M., Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
ATP synthesis in cell-free extracts of peanut (Arachis hypogaea L.) seeds
Cell-free extracts of peanut (Arachis hypogaea L., cv. Shulamit) seeds, incubated with various substrates, synthesized ATP. Significant synthesis occurred in the presence of AMP + PEP, NADH 2 + PEP and NAD + PEP. When the activities were examined in extracts prepared with 0.3 M mannitol, the rates were 0.6, 0.1 and 0.04 nmol min -1 mg -1 protein, respectively. The activities under such conditions were linear with time up to 90 min incubation at 30 °C. In the presence of PEP + NADH 2 there was a higher specific activity in extracts from non-dormant seeds than from dormant seeds. No such difference was found when PEP + AMP or NAD + PEP was used as the substrate. The temperature dependence of the activity showed a relatively high energy of activation (Ea) for AMP + PEP and a low one if NADH 2 + PEP or NAD + PEP was used as substrate. In buffer extracts of seeds ATP was synthesized in the presence of the above-mentioned substrate combinations but the rate of activity exhibited a lag phase at the early time of incubation, after which higher rates of activities (as compared with mannitol extracts) were obtained. The activities were Co +-dependent, with a K m of about 0.7 mM. In the buffer extracts relatively high activities of adenylate kinase (EC 2.7.4.3 (AK) and pyruvate kinase (EC 2.7.1.50) (PK) were found. AK was stimulated by ethephon (ethylene). This effect is temperature-dependent and occurs in both directions: in the presence of ADP (→ATP + AMP) as well as if ATP + AMP is used as substrate to synthesize ADP. PK is Co +-dependent, and unaffected by ethephon. Both activities were stimulated by malonate. © 1982 Oxford University Press.
Scientific Publication
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