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פותח על ידי קלירמאש פתרונות בע"מ -
Oxidative enzymes, ribonuclease, and amylase in lemon buds infested with Aceria sheldoni (ewing) (acarina: Eriophyidae)
Year:
1971
Source of publication :
Journal of Experimental Botany
Authors :
ישעיה, יצחק
;
.
Volume :
22
Co-Authors:
Ishaaya, I., Division of Entomology, The Volcani Institute of Agricultural Research, Bet Dagan, Israel
Sternlicht, M., Division of Entomology, The Volcani Institute of Agricultural Research, Bet Dagan, Israel
Facilitators :
From page:
146
To page:
152
(
Total pages:
7
)
Abstract:
In lemon buds the optimum for peroxidase activity was a reaction mixture of 0.03 M guiacol, 0.1 per cent enzyme (acetone powder), and 0.0086 per cent H2O2, in 0.015 M acetate buffer pH 4.0. For polyphenoloxidase activity the optimum was a reaction mixture of 0.3 per cent catechol and 0.025 per cent enzyme (acetone powder), in 0.05 M phosphate buffer pH 7.0. Both enzymes were strongly affected by mite infestation. The activity of polyphenoloxidase and peroxidase in infested buds reaches respectively two and three times that in uninfested buds. There are indications that the enhanced oxidative activity and corresponding increase in phenol level in the infested buds is part of a defence system which may develop in the plant after infestation.The optimum for RNAase activity was a reaction mixture of 0.06 per cent RNA and 0.02 per cent purified enzyme, in 0.035 M sodium potassium-buffer pH 5.6. An increase of about 30 per cent in RNAase activity occurred after mite infestation. Amylase activity was affected slightly only after a heavy infestation. © 1971 Oxford University Press.
Note:
Related Files :
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More details
DOI :
10.1093/jxb/22.1.146
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32224
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:08
Scientific Publication
Oxidative enzymes, ribonuclease, and amylase in lemon buds infested with Aceria sheldoni (ewing) (acarina: Eriophyidae)
22
Ishaaya, I., Division of Entomology, The Volcani Institute of Agricultural Research, Bet Dagan, Israel
Sternlicht, M., Division of Entomology, The Volcani Institute of Agricultural Research, Bet Dagan, Israel
Oxidative enzymes, ribonuclease, and amylase in lemon buds infested with Aceria sheldoni (ewing) (acarina: Eriophyidae)
In lemon buds the optimum for peroxidase activity was a reaction mixture of 0.03 M guiacol, 0.1 per cent enzyme (acetone powder), and 0.0086 per cent H2O2, in 0.015 M acetate buffer pH 4.0. For polyphenoloxidase activity the optimum was a reaction mixture of 0.3 per cent catechol and 0.025 per cent enzyme (acetone powder), in 0.05 M phosphate buffer pH 7.0. Both enzymes were strongly affected by mite infestation. The activity of polyphenoloxidase and peroxidase in infested buds reaches respectively two and three times that in uninfested buds. There are indications that the enhanced oxidative activity and corresponding increase in phenol level in the infested buds is part of a defence system which may develop in the plant after infestation.The optimum for RNAase activity was a reaction mixture of 0.06 per cent RNA and 0.02 per cent purified enzyme, in 0.035 M sodium potassium-buffer pH 5.6. An increase of about 30 per cent in RNAase activity occurred after mite infestation. Amylase activity was affected slightly only after a heavy infestation. © 1971 Oxford University Press.
Scientific Publication
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