Vengadesan, G., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India, Hardwood Tree Improvement and Regeneration Center, Department of Forestry and Natural Resources, Purdue University, 715, West State Street, West Lafayette, IN 47907-2061, United States
Amutha, S., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India, Agriculture Research Organization, Institute for Plant Protection, Department of Virology, POB 6, Bet Dagan 50250, Israel
Muruganantham, M., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India, Agriculture Research Organization, Institute for Plant Protection, Department of Virology, POB 6, Bet Dagan 50250, Israel
Anand, R.P., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India, Molecular Plant Virology Laboratory, Department of Plant Pathology, National Chung Hsing University, Taicung, Taiwan
Ganapathi, A., Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India

Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 μM acetosyringone and shoots regenerated on MS (Murashige and Skoog, 1962, Physiol Plant 15:473-497) medium with 13.3 μM benzylaminopurine, 2.6 μM indole-3-acetic acid, 1 g l-1 activated charcoal, 1.5 mg l-1 phosphinothricin, and 300 mg l -1 cefotaxime. Phosphinothricin at 1.5 mg l-1 was used for the selection. Shoots surviving selection on medium with phosphinothricin expressed GUS. Following Southern hybridization, eight independent shoots regenerated of 500 cocultivated explants were demonstrated to be transgenic, which represented transformation frequency of 1.6%. The transgenics carried one to four copies of the transgene. Transgenic shoots were propagated as microcuttings in MS medium with 6.6 μM 6-benzylaminopurine and 1.5 mg l -1 phosphinothricin. Shoots elongated and rooted in MS medium with gibberellic acid and indole-3-butyric acid, respectively both supplemented with 1.5 mg l-1 phosphinothricin. Micropropagation of transgenic plants by microcuttings proved to be a simple means to bulk up the material. Several transgenic plants were found to be resistant to leaf painting with the herbicide Basta. © 2006 Springer-Verlag.