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פותח על ידי קלירמאש פתרונות בע"מ -
An immortalized rat liver stellate cell line (HSC-T6): A new cell model for the study of retinoid metabolism in vitro
Year:
2000
Source of publication :
Journal of lipid research
Authors :
לאלזר, אברהם
;
.
Volume :
41
Co-Authors:
Vogel, S., Department of Medicine, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
Piantedosi, R., Department of Medicine, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
Frank, J., Department of Dermatology, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
Lalazar, A., Division of Liver Diseases, Mount Sinai School of Medicine, New York, NY 10029, United States
Rockey, D.C., Duke Liver Center, Duke University Medical Center, Durham, NC 27710, United States
Friedman, S.L., Division of Liver Diseases, Mount Sinai School of Medicine, New York, NY 10029, United States
Blaner, W.S., Department of Medicine, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States, Institute of Human Nutrition, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
Facilitators :
From page:
882
To page:
893
(
Total pages:
12
)
Abstract:
Hepatocytes and hepatic stellate cells play important roles in retinoid storage and metabolism. Hepatocytes process postprandial retinyl esters and are responsible for secretion of retinol bound to retinol-binding protein (RBP) to maintain plasma retinol levels. Stellate cells are the body's major cellular storage sites for retinoid. We have characterized and utilized an immortalized rat stellate cell line, HSC-T6 cells, to facilitate study of the cellular aspects of hepatic retinoid processing. For comparison, we also carried out parallel studies in Hepa-1 hepatocytes. Like activated primary stellate cells, HSC-T6 express myogenic and neural crest cytoskeletal filaments. HSC-T6 cells take up and esterify retinol in a time- and concentration-dependent manner. Supplementation of HSC-T6 culture medium with free fatty acids (up to 300 μM) does not affect retinol uptake but does enhance retinol esterification up to 10-fold. RT-PCR analysis indicates that HSC-T6 cells express all 6 retinoid nuclear receptors (RARα, -β, -γ, and RXRα, -β, -γ) and like primary stellate cells, HSC-T6 stellate cells express cellular retinol-binding protein, type I (CRBP) but fail to express either retinol-binding protein (RBP) or transthyretin (TTR). Addition of retinol (10-8-10-5 M) or all-trans-retinoic acid (10-10-10-6 M) rapidly up-regulates CRBP expression. Using RAR-specific agonists and antagonists and an RXR-specific agonist, we show that members of the RAR- receptor family modulate HSC-T6 CRBP expression. Thus, HSC-T6 cells display the same retinoid-related phenotype as primary stellate cells in culture and will be a useful tool for study of hepatic retinoid storage and metabolism.
Note:
Related Files :
animal cell
Animals
Cell Line, Transformed
Fat-storing cells
Hepa-1 hepatocytes
liver metabolism
Male
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32316
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:08
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Scientific Publication
An immortalized rat liver stellate cell line (HSC-T6): A new cell model for the study of retinoid metabolism in vitro
41
Vogel, S., Department of Medicine, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
Piantedosi, R., Department of Medicine, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
Frank, J., Department of Dermatology, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
Lalazar, A., Division of Liver Diseases, Mount Sinai School of Medicine, New York, NY 10029, United States
Rockey, D.C., Duke Liver Center, Duke University Medical Center, Durham, NC 27710, United States
Friedman, S.L., Division of Liver Diseases, Mount Sinai School of Medicine, New York, NY 10029, United States
Blaner, W.S., Department of Medicine, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States, Institute of Human Nutrition, Coll. Phys. Surgs. of Columbia Univ., New York, NY 10032, United States
An immortalized rat liver stellate cell line (HSC-T6): A new cell model for the study of retinoid metabolism in vitro
Hepatocytes and hepatic stellate cells play important roles in retinoid storage and metabolism. Hepatocytes process postprandial retinyl esters and are responsible for secretion of retinol bound to retinol-binding protein (RBP) to maintain plasma retinol levels. Stellate cells are the body's major cellular storage sites for retinoid. We have characterized and utilized an immortalized rat stellate cell line, HSC-T6 cells, to facilitate study of the cellular aspects of hepatic retinoid processing. For comparison, we also carried out parallel studies in Hepa-1 hepatocytes. Like activated primary stellate cells, HSC-T6 express myogenic and neural crest cytoskeletal filaments. HSC-T6 cells take up and esterify retinol in a time- and concentration-dependent manner. Supplementation of HSC-T6 culture medium with free fatty acids (up to 300 μM) does not affect retinol uptake but does enhance retinol esterification up to 10-fold. RT-PCR analysis indicates that HSC-T6 cells express all 6 retinoid nuclear receptors (RARα, -β, -γ, and RXRα, -β, -γ) and like primary stellate cells, HSC-T6 stellate cells express cellular retinol-binding protein, type I (CRBP) but fail to express either retinol-binding protein (RBP) or transthyretin (TTR). Addition of retinol (10-8-10-5 M) or all-trans-retinoic acid (10-10-10-6 M) rapidly up-regulates CRBP expression. Using RAR-specific agonists and antagonists and an RXR-specific agonist, we show that members of the RAR- receptor family modulate HSC-T6 CRBP expression. Thus, HSC-T6 cells display the same retinoid-related phenotype as primary stellate cells in culture and will be a useful tool for study of hepatic retinoid storage and metabolism.
Scientific Publication
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