חיפוש מתקדם
Experimental Mycology
Freeman, S., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan 50250, United States
Pham, M., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan 50250, United States
Rodriguez, R.J., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan 50250, United States
Isolates of Colletotrichum were grouped into 10 separate species based on arbitrarily primed PCR (ap-PCR), A + T-rich DNA (AT-DNA) and nuclear DNA banding patterns. In general, the grouping of Colletotrichum isolates by these molecular approaches corresponded to that done by classical taxonomic identification, however, some exceptions were observed. PCR amplification of genomic DNA using four different primers allowed for reliable differentiation between isolates of the 10 species. HaeIII digestion patterns of AT-DNA also distinguished between species of Colletotrichum by generating species-specific band patterns. In addition, hybridization of the repetitive DNA element (GcpR1) to genomic DNA identified a unique set of PstI-digested nuclear DNA fragments in each of the 10 species of Colletotrichum tested. Multiple isolates of C. acutatum, C. coccodes, C. fragariae, C. lindemuthianum, C. magna, C. orbiculare, C. graminicola from maize, and C. graminicola from sorghum showed 86-100% intraspecies similarity based on ap-PCR and AT-DNA analyses. Interspecies similarity determined by ap-PCR and AT-DNA analyses varied between 0 and 33%. Three distinct banding patterns were detected in isolates of C. gloeosporioides from strawberry. Similarly, three different banding patterns were observed among isoltes of C. musae from diseased banana.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Molecular genotyping of colletotrichum species based on arbitrarily primed PCR, A + T-rich DNA, and nuclear DNA analyses
17
Freeman, S., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan 50250, United States
Pham, M., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan 50250, United States
Rodriguez, R.J., Department of Plant Pathology, ARO, The Volcani Center, Bet Dagan 50250, United States
Molecular genotyping of colletotrichum species based on arbitrarily primed PCR, A + T-rich DNA, and nuclear DNA analyses
Isolates of Colletotrichum were grouped into 10 separate species based on arbitrarily primed PCR (ap-PCR), A + T-rich DNA (AT-DNA) and nuclear DNA banding patterns. In general, the grouping of Colletotrichum isolates by these molecular approaches corresponded to that done by classical taxonomic identification, however, some exceptions were observed. PCR amplification of genomic DNA using four different primers allowed for reliable differentiation between isolates of the 10 species. HaeIII digestion patterns of AT-DNA also distinguished between species of Colletotrichum by generating species-specific band patterns. In addition, hybridization of the repetitive DNA element (GcpR1) to genomic DNA identified a unique set of PstI-digested nuclear DNA fragments in each of the 10 species of Colletotrichum tested. Multiple isolates of C. acutatum, C. coccodes, C. fragariae, C. lindemuthianum, C. magna, C. orbiculare, C. graminicola from maize, and C. graminicola from sorghum showed 86-100% intraspecies similarity based on ap-PCR and AT-DNA analyses. Interspecies similarity determined by ap-PCR and AT-DNA analyses varied between 0 and 33%. Three distinct banding patterns were detected in isolates of C. gloeosporioides from strawberry. Similarly, three different banding patterns were observed among isoltes of C. musae from diseased banana.
Scientific Publication
You may also be interested in