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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The use of short and long PCR products for improved detection of prunus necrotic ringspot virus in woody plants
Year:
1997
Source of publication :
Journal of Virological Methods
Authors :
מסלנין, לודמילה
;
.
רוזנר, אריה
;
.
שפיגל, שרה
;
.
Volume :
67
Co-Authors:
Rosner, A., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Facilitators :
From page:
135
To page:
141
(
Total pages:
7
)
Abstract:
The reverse transcriptase-polymerase chain reaction (RT-PCR) was used for detection of prunus necrotic ringspot virus (PNRSV) in dormant peach and almond trees by the application of two different pairs of primers yielding a short and a long product, respectively. The relative amount of the short (200 base pair, bp) product was higher than the longer (785 bp) product. PNRSV was detected better in plant tissues with a low virus concentration (e.g. dormant trees) by amplification of the short PCR product, whereas the long product was produced at higher virus titers. Simultaneous amplification of both short and long products was demonstrated using a three-primer mixture in a single reaction tube. In this assay, amplification of either PCR product indicated the presence of PNRSV-specific sequences in the plant tissue examined, thus covering a wide range of virus concentrations in a single test. Dilution of the RNA extracted from infected plant material resulted in a steep decline in the amplification of both short and long PCR products. In contrast, serial dilutions of the intermediate cDNA template differentially affected the amplification patterns: the relative amount of the short product increased whereas that of the long product decreased. These results may explain the preferential amplification of the short PCR product observed in samples containing low virus concentrations.
Note:
Related Files :
Prunus
Prunus persica
stone fruits
Templates, Genetic
trees
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0166-0934(97)00088-8
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32403
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:09
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Scientific Publication
The use of short and long PCR products for improved detection of prunus necrotic ringspot virus in woody plants
67
Rosner, A., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
The use of short and long PCR products for improved detection of prunus necrotic ringspot virus in woody plants
The reverse transcriptase-polymerase chain reaction (RT-PCR) was used for detection of prunus necrotic ringspot virus (PNRSV) in dormant peach and almond trees by the application of two different pairs of primers yielding a short and a long product, respectively. The relative amount of the short (200 base pair, bp) product was higher than the longer (785 bp) product. PNRSV was detected better in plant tissues with a low virus concentration (e.g. dormant trees) by amplification of the short PCR product, whereas the long product was produced at higher virus titers. Simultaneous amplification of both short and long products was demonstrated using a three-primer mixture in a single reaction tube. In this assay, amplification of either PCR product indicated the presence of PNRSV-specific sequences in the plant tissue examined, thus covering a wide range of virus concentrations in a single test. Dilution of the RNA extracted from infected plant material resulted in a steep decline in the amplification of both short and long PCR products. In contrast, serial dilutions of the intermediate cDNA template differentially affected the amplification patterns: the relative amount of the short product increased whereas that of the long product decreased. These results may explain the preferential amplification of the short PCR product observed in samples containing low virus concentrations.
Scientific Publication
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