Co-Authors:
Fuchs, Y., The Volcani Institute of Agricultural Research, Bet Dagan, Israel
Mayak, S., The Volcani Institute of Agricultural Research, Bet Dagan, Israel
Fuchs, S., Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel
Abstract:
Antibodies with specificity towards abscisic acid (ABA) were produced in rabbits. These antibodies were used for assaying ABA by the inhibition of inactivation of modified bacteriophage. For this assay conjugates of ABA with bacteriophage T4 were prepared and characterized. Such chemically modified bacteriophages were completely inactivated by the specific anti-ABA serum and this inactivation was inhibited by free ABA. The identification and quantitative determination of ABA in plant extracts by this method are demonstrated and the method is compared with a common bioassay. © 1972 Springer-Verlag.