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Current Microbiology
Zhou, H., Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States
Yao, F., Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States
Roberts, D.P., Sustainable Agric. Syst. Laboratory, USDA-ARS, Bldg. 001, 10300 Baltimore Ave., Beltsville, MD 20705-2350, United States
Lessie, T.G., Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States
Burkholderia ambifaria BC-F, a biocontrol strain reported previously to exhibit broad-spectrum antifungal activity, was highly active in formation of N-acyl homoserine lactones (AHLs). We constructed AHL-deficient derivatives of strain BC-F in which the genes specifying AHL synthase (bafI) and AHL-binding transcriptional activator (bafR) were inactivated by allelic exchange. The resulting AHL-deficient mutants had decreased antifungal activity.
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AHL-deficient mutants of Burkholderia ambifaria BC-F have decreased antifungal activity
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Zhou, H., Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States
Yao, F., Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States
Roberts, D.P., Sustainable Agric. Syst. Laboratory, USDA-ARS, Bldg. 001, 10300 Baltimore Ave., Beltsville, MD 20705-2350, United States
Lessie, T.G., Department of Microbiology, University of Massachusetts, Amherst, MA 01003, United States
AHL-deficient mutants of Burkholderia ambifaria BC-F have decreased antifungal activity
Burkholderia ambifaria BC-F, a biocontrol strain reported previously to exhibit broad-spectrum antifungal activity, was highly active in formation of N-acyl homoserine lactones (AHLs). We constructed AHL-deficient derivatives of strain BC-F in which the genes specifying AHL synthase (bafI) and AHL-binding transcriptional activator (bafR) were inactivated by allelic exchange. The resulting AHL-deficient mutants had decreased antifungal activity.
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