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פותח על ידי קלירמאש פתרונות בע"מ -
Potato spindle tuber viroid-infected tissues contain RNA complementary to the entire viroid
Year:
1982
Source of publication :
Journal of General Virology
Authors :
זלצר, אהרון
;
.
Volume :
59
Co-Authors:
Zelcer, A., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Zaitlin, M., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Robertson, H.D., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Dickson, E., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Facilitators :
From page:
139
To page:
148
(
Total pages:
10
)
Abstract:
Potato spindle tuber viroid (PSTV) RNA, labelled in vitro with 125I, was hybridized in solution to RNA prepared from uninfected and PSTV-infected Rutgers tomato plants and suspension, cultures. Following hybridization to RNA from infected plants, 125I-labelled PSTV was converted from its single-stranded form to double-stranded RNA; this onversion did not occur to a significant extent when the 125I-labelled PSTV was incubated with RNA from uninfected tomato plants under identical conditions. Following fractionation of RNA from PSTV-infected tissue with 2 M-LiCl and chromatography on cellulose CF11 columns, the RNA species which hybridizes with the PSTV probe was found to be enriched in those fractions which are also enriched for double-stranded RNA. Fingerprint analysis of hybridized 125I-labelled PSTV following recovery from the hybrids demonstrated that all regions of the viroid are represented in the complementary strands present in these RNA preparations.
Note:
Related Files :
animal experiment
higher plant
Potato spindle tuber viroid
virus infection
virus RNA
עוד תגיות
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DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
Language:
אנגלית
Editors' remarks:
ID:
32469
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:10
Scientific Publication
Potato spindle tuber viroid-infected tissues contain RNA complementary to the entire viroid
59
Zelcer, A., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Zaitlin, M., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Robertson, H.D., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Dickson, E., Dep. Plant Pathol., Cornell Univ., Ithaca, NY 14853, United States
Potato spindle tuber viroid-infected tissues contain RNA complementary to the entire viroid
Potato spindle tuber viroid (PSTV) RNA, labelled in vitro with 125I, was hybridized in solution to RNA prepared from uninfected and PSTV-infected Rutgers tomato plants and suspension, cultures. Following hybridization to RNA from infected plants, 125I-labelled PSTV was converted from its single-stranded form to double-stranded RNA; this onversion did not occur to a significant extent when the 125I-labelled PSTV was incubated with RNA from uninfected tomato plants under identical conditions. Following fractionation of RNA from PSTV-infected tissue with 2 M-LiCl and chromatography on cellulose CF11 columns, the RNA species which hybridizes with the PSTV probe was found to be enriched in those fractions which are also enriched for double-stranded RNA. Fingerprint analysis of hybridized 125I-labelled PSTV following recovery from the hybrids demonstrated that all regions of the viroid are represented in the complementary strands present in these RNA preparations.
Scientific Publication
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