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A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus
Year:
1990
Source of publication :
Virology
Authors :
רקח, בנימין
;
.
Volume :
178
Co-Authors:
Atreya, C.D., Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, United States
Raccah, B., Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, United States
Pirone, T.P., Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, United States
Facilitators :
From page:
161
To page:
165
(
Total pages:
5
)
Abstract:
A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of TVMV-NAT revealed a single nucleotide difference (G → A) at position 8445; this alters a single amino acid residue (G → E) at position 2747. A cDNA fragment representing the CP region of TVMV-NAT was substituted into the CP region of a full-length cDNA clone of TVMV-AT, and transcribed RNA was inoculated to tobacco plants. Aphids were unable to transmit the resultant hybrid virus which had the TVMV-NAT coat protein, although the concentration and infectivity of the hybrid virus in the source plants were similar to those of TVMV-AT. This is the first direct demonstration that a CP mutation affects aphid transmissibility of a potyvirus. © 1990.
Note:
Related Files :
Base Sequence
genetic engineering
Molecular Sequence Data
mutation
Nicotiana obtusifolia
Plants, Toxic
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0042-6822(90)90389-9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32500
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:10
You may also be interested in
Scientific Publication
A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus
178
Atreya, C.D., Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, United States
Raccah, B., Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, United States
Pirone, T.P., Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, United States
A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus
A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of TVMV-NAT revealed a single nucleotide difference (G → A) at position 8445; this alters a single amino acid residue (G → E) at position 2747. A cDNA fragment representing the CP region of TVMV-NAT was substituted into the CP region of a full-length cDNA clone of TVMV-AT, and transcribed RNA was inoculated to tobacco plants. Aphids were unable to transmit the resultant hybrid virus which had the TVMV-NAT coat protein, although the concentration and infectivity of the hybrid virus in the source plants were similar to those of TVMV-AT. This is the first direct demonstration that a CP mutation affects aphid transmissibility of a potyvirus. © 1990.
Scientific Publication
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