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פותח על ידי קלירמאש פתרונות בע"מ -
Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein
Year:
2008
Authors :
בלאוסוב, אדוארד
;
.
גדעוני, דוד
;
.
גליק, אפרת
;
.
גפני, ידידיה
;
.
זרחיה, אבי
;
.
לוי, יעל
;
.
מט, אנהיט
;
.
Volume :
105
Co-Authors:
Glick, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Zrachya, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Levy, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Mett, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Gidoni, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Citovsky, V., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Gafni, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
157
To page:
161
(
Total pages:
5
)
Abstract:
The V2 protein of tomato yellow leaf curl geminivirus (TYLCV) functions as an RNA-silencing suppressor that counteracts the innate immune response of the host plant. The host-cell target of V2, however, remains unknown. Here we show that V2 interacts directly with SlSGS3, the tomato homolog of the Arabidopsis SGS3 protein (AtSGS3), which is known to be involved in the RNA-silencing pathway. SlSGS3 genetically complemented an AtSGS3 mutation and restored RNA silencing, indicating that SlSGS3 is indeed a functional homolog of AtSGS3. A point mutant of V2 that is unable to bind SlSGS3 also lost its ability to suppress RNA silencing, suggesting a correlation between the V2-SlSGS3 interaction in planta and the suppressor activity of V2. © 2007 by The National Academy of Sciences of the USA.
Note:
Related Files :
arabidopsis
gene silencing
mutation
RNA
Tomato yellow leaf curl virus
v2 protein
עוד תגיות
תוכן קשור
More details
DOI :
10.1073/pnas.0709036105
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32503
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:10
Scientific Publication
Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein
105
Glick, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Zrachya, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Levy, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Mett, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Gidoni, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Citovsky, V., Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Gafni, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P. O. Box 6, Bet Dagan 50250, Israel
Interaction with host SGS3 is required for suppression of RNA silencing by tomato yellow leaf curl virus V2 protein
The V2 protein of tomato yellow leaf curl geminivirus (TYLCV) functions as an RNA-silencing suppressor that counteracts the innate immune response of the host plant. The host-cell target of V2, however, remains unknown. Here we show that V2 interacts directly with SlSGS3, the tomato homolog of the Arabidopsis SGS3 protein (AtSGS3), which is known to be involved in the RNA-silencing pathway. SlSGS3 genetically complemented an AtSGS3 mutation and restored RNA silencing, indicating that SlSGS3 is indeed a functional homolog of AtSGS3. A point mutant of V2 that is unable to bind SlSGS3 also lost its ability to suppress RNA silencing, suggesting a correlation between the V2-SlSGS3 interaction in planta and the suppressor activity of V2. © 2007 by The National Academy of Sciences of the USA.
Scientific Publication
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