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פותח על ידי קלירמאש פתרונות בע"מ -
Expression of foreign genes in chicks by hydrodynamics-based naked plasmid transfer in vivo
Year:
2006
Source of publication :
Domestic Animal Endocrinology
Authors :
דרוין, שלי
;
.
יהב, שלמה
;
.
שמחייב, ויקטוריה
;
.
Volume :
30
Co-Authors:
Hen, G., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Bor, A., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Simchaev, V., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Druyan, S., Department of Genetics, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University, Rehovot 76-100, Israel
Yahav, S., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Miao, C.H., Department of Medicine, University of Washington, Seattle, WA 98195, United States
Friedman-Einat, M., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Facilitators :
From page:
135
To page:
143
(
Total pages:
9
)
Abstract:
The study of gene function in vivo is considered one of the top achievements of modern biology, inasmuch as it provides tools to study gene function in the context of the whole animal. In chickens, techniques of DNA-mediated gene transfer are less advanced than in other animal or livestock models, and remain a significant challenge. The study presented here is the first to show that a hydrodynamics-based gene-transfer technique, originally developed for naked DNA transfer in mice, can be applied to chickens. Rapid injection of naked plasmids containing expression cassettes into the jugular vein of 6- to 10-day-old chicks resulted in specific expression of the transgenes. A CMV promoter-driven luciferase reporter gene was expressed at significant levels in the liver during the first 3 days post-injection with lower levels also detected in the kidney. Significantly, all injected birds showed detectable levels of luciferase expression. Similarly, injection of a plasmid containing the secreted human coagulation factor IX (hFIX) gene under the control of human alpha-1-anti-trypsin promoter resulted in detectable levels of the hFIX in the plasma during the first 2 days post-injection. The method described herein has the potential for a quick and simple route for gain and loss-of function experiments in chicken liver and kidney, as well as for studying systemic effects of secreted proteins and hormones. © 2005 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animal
Animals
Chickens
Cucumber mosaic virus
Genetics
gene transfer
liver
metabolism
Plasmid
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.domaniend.2005.06.002
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32541
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:10
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Scientific Publication
Expression of foreign genes in chicks by hydrodynamics-based naked plasmid transfer in vivo
30
Hen, G., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Bor, A., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Simchaev, V., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Druyan, S., Department of Genetics, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University, Rehovot 76-100, Israel
Yahav, S., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Miao, C.H., Department of Medicine, University of Washington, Seattle, WA 98195, United States
Friedman-Einat, M., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Expression of foreign genes in chicks by hydrodynamics-based naked plasmid transfer in vivo
The study of gene function in vivo is considered one of the top achievements of modern biology, inasmuch as it provides tools to study gene function in the context of the whole animal. In chickens, techniques of DNA-mediated gene transfer are less advanced than in other animal or livestock models, and remain a significant challenge. The study presented here is the first to show that a hydrodynamics-based gene-transfer technique, originally developed for naked DNA transfer in mice, can be applied to chickens. Rapid injection of naked plasmids containing expression cassettes into the jugular vein of 6- to 10-day-old chicks resulted in specific expression of the transgenes. A CMV promoter-driven luciferase reporter gene was expressed at significant levels in the liver during the first 3 days post-injection with lower levels also detected in the kidney. Significantly, all injected birds showed detectable levels of luciferase expression. Similarly, injection of a plasmid containing the secreted human coagulation factor IX (hFIX) gene under the control of human alpha-1-anti-trypsin promoter resulted in detectable levels of the hFIX in the plasma during the first 2 days post-injection. The method described herein has the potential for a quick and simple route for gain and loss-of function experiments in chicken liver and kidney, as well as for studying systemic effects of secreted proteins and hormones. © 2005 Elsevier Inc. All rights reserved.
Scientific Publication
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