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Domestic Animal Endocrinology
Hen, G., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Bor, A., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Simchaev, V., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Druyan, S., Department of Genetics, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University, Rehovot 76-100, Israel
Yahav, S., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Miao, C.H., Department of Medicine, University of Washington, Seattle, WA 98195, United States
Friedman-Einat, M., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
The study of gene function in vivo is considered one of the top achievements of modern biology, inasmuch as it provides tools to study gene function in the context of the whole animal. In chickens, techniques of DNA-mediated gene transfer are less advanced than in other animal or livestock models, and remain a significant challenge. The study presented here is the first to show that a hydrodynamics-based gene-transfer technique, originally developed for naked DNA transfer in mice, can be applied to chickens. Rapid injection of naked plasmids containing expression cassettes into the jugular vein of 6- to 10-day-old chicks resulted in specific expression of the transgenes. A CMV promoter-driven luciferase reporter gene was expressed at significant levels in the liver during the first 3 days post-injection with lower levels also detected in the kidney. Significantly, all injected birds showed detectable levels of luciferase expression. Similarly, injection of a plasmid containing the secreted human coagulation factor IX (hFIX) gene under the control of human alpha-1-anti-trypsin promoter resulted in detectable levels of the hFIX in the plasma during the first 2 days post-injection. The method described herein has the potential for a quick and simple route for gain and loss-of function experiments in chicken liver and kidney, as well as for studying systemic effects of secreted proteins and hormones. © 2005 Elsevier Inc. All rights reserved.
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תנאי שימוש
Expression of foreign genes in chicks by hydrodynamics-based naked plasmid transfer in vivo
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Hen, G., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Bor, A., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Simchaev, V., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Druyan, S., Department of Genetics, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University, Rehovot 76-100, Israel
Yahav, S., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Miao, C.H., Department of Medicine, University of Washington, Seattle, WA 98195, United States
Friedman-Einat, M., Department of Animal Science, Agricultural Research Organization, Volcani Center, Derech Hamacabim st., Bet Dagan 50-250, Israel
Expression of foreign genes in chicks by hydrodynamics-based naked plasmid transfer in vivo
The study of gene function in vivo is considered one of the top achievements of modern biology, inasmuch as it provides tools to study gene function in the context of the whole animal. In chickens, techniques of DNA-mediated gene transfer are less advanced than in other animal or livestock models, and remain a significant challenge. The study presented here is the first to show that a hydrodynamics-based gene-transfer technique, originally developed for naked DNA transfer in mice, can be applied to chickens. Rapid injection of naked plasmids containing expression cassettes into the jugular vein of 6- to 10-day-old chicks resulted in specific expression of the transgenes. A CMV promoter-driven luciferase reporter gene was expressed at significant levels in the liver during the first 3 days post-injection with lower levels also detected in the kidney. Significantly, all injected birds showed detectable levels of luciferase expression. Similarly, injection of a plasmid containing the secreted human coagulation factor IX (hFIX) gene under the control of human alpha-1-anti-trypsin promoter resulted in detectable levels of the hFIX in the plasma during the first 2 days post-injection. The method described herein has the potential for a quick and simple route for gain and loss-of function experiments in chicken liver and kidney, as well as for studying systemic effects of secreted proteins and hormones. © 2005 Elsevier Inc. All rights reserved.
Scientific Publication
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