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פותח על ידי קלירמאש פתרונות בע"מ -
Prevention of liver cirrhosis in rats by curcumin
Year:
2007
Source of publication :
Liver International
Authors :
גנין, אולגה
;
.
פינס, מרק
;
.
Volume :
27
Co-Authors:
Bruck, R., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel, The Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Ashkenazi, M., The Unit of Clinical Nutrition, The E. Wolfson Medical Center, Holon, Israel
Weiss, S., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel
Goldiner, I., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel
Shapiro, H., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel, Department of Hypnosis, The E. Wolfson Medical Center, Holon, Israel
Aeed, H., Department of Gastroenterology, The E. Wolfson Medical Center, Holon, Israel
Genina, O., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel
Helpern, Z., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel, The Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Pines, M., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
373
To page:
383
(
Total pages:
11
)
Abstract:
Background and Aim: Curcumin, the major polyphenolic compound in turmeric, has been shown to attenuate hepatic damage in several animal models of liver injury. The aim of the present study was to examine the efficacy of curcumin in preventing thioacetamide-induced cirrhosis and to unravel the mechanism of curcumin's effect on hepatic fibrosis in rats. Methods: Liver cirrhosis was induced by thioacetamide (TAA; 200 mg/kg, i.p.) twice weekly for 12 weeks. One group of rats concomitantly received curcumin (300 mg/kg/day, by gavage for 12 weeks); the control group received the solvent at identical amounts and duration. Results: TAA administration induced liver cirrhosis, which was inhibited by curcumin. Liver histopathology, hydroxyproline levels and spleen weights were significantly lower in the rats treated with TAA+curcumin compared with TAA only (P < 0.001). Immunohistochemical studies and in situ hybridization demonstrated inhibition of hepatic stellate cell (α smooth muscle actin-positive) activation and collagen αI (I) gene expression in the livers of the TAA+curcumin-treated rats. Curcumin reduced oxidative stress as shown by the decreased hepatic nitrotyrosine staining in the curcumin+TAA-treated rats. Curcumin treatment had no effect on pre existing liver cirrhosis. As determined by in vitro studies using the rat HSC-T6 cell line, curcumin had no direct inhibitory effect on collagen α1 (I) messenger RNA expression. Further studies in these cells using reverse transcriptase-polymerase chain reaction demonstrated that curcumin had no effect on the expression of PDGF-induced TIMP-1 and TIMP-2, TGFβ1, TGFβ2 and MCP-1 but significantly inhibited tumor necrosis factor alpha expression. Curcumin had no effect on hepatic stellate cells proliferation. Zymography showed that curcumin had no effect on matrix metalloproteinase-2 activity. Conclusions: Curcumin inhibited the development of TAA-induced liver cirrhosis mainly due to its anti-inflammatory activities and not by a direct anti-fibrotic effect. As curcumin ingestion is safe in humans, it may be reasonable to assess in clinical studies the beneficial effect of curcumin in slowing the development of liver cirrhosis. © 2007 Blackwell Munksgaard.
Note:
Related Files :
animal experiment
Animals
Cell Proliferation
gene expression
hydroxyproline
Male
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1478-3231.2007.01453.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32556
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:10
You may also be interested in
Scientific Publication
Prevention of liver cirrhosis in rats by curcumin
27
Bruck, R., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel, The Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Ashkenazi, M., The Unit of Clinical Nutrition, The E. Wolfson Medical Center, Holon, Israel
Weiss, S., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel
Goldiner, I., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel
Shapiro, H., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel, Department of Hypnosis, The E. Wolfson Medical Center, Holon, Israel
Aeed, H., Department of Gastroenterology, The E. Wolfson Medical Center, Holon, Israel
Genina, O., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel
Helpern, Z., The Department of Gastroenterology, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, Tel-Aviv 64239, Israel, The Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Pines, M., Institute of Animal Science, Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel
Prevention of liver cirrhosis in rats by curcumin
Background and Aim: Curcumin, the major polyphenolic compound in turmeric, has been shown to attenuate hepatic damage in several animal models of liver injury. The aim of the present study was to examine the efficacy of curcumin in preventing thioacetamide-induced cirrhosis and to unravel the mechanism of curcumin's effect on hepatic fibrosis in rats. Methods: Liver cirrhosis was induced by thioacetamide (TAA; 200 mg/kg, i.p.) twice weekly for 12 weeks. One group of rats concomitantly received curcumin (300 mg/kg/day, by gavage for 12 weeks); the control group received the solvent at identical amounts and duration. Results: TAA administration induced liver cirrhosis, which was inhibited by curcumin. Liver histopathology, hydroxyproline levels and spleen weights were significantly lower in the rats treated with TAA+curcumin compared with TAA only (P < 0.001). Immunohistochemical studies and in situ hybridization demonstrated inhibition of hepatic stellate cell (α smooth muscle actin-positive) activation and collagen αI (I) gene expression in the livers of the TAA+curcumin-treated rats. Curcumin reduced oxidative stress as shown by the decreased hepatic nitrotyrosine staining in the curcumin+TAA-treated rats. Curcumin treatment had no effect on pre existing liver cirrhosis. As determined by in vitro studies using the rat HSC-T6 cell line, curcumin had no direct inhibitory effect on collagen α1 (I) messenger RNA expression. Further studies in these cells using reverse transcriptase-polymerase chain reaction demonstrated that curcumin had no effect on the expression of PDGF-induced TIMP-1 and TIMP-2, TGFβ1, TGFβ2 and MCP-1 but significantly inhibited tumor necrosis factor alpha expression. Curcumin had no effect on hepatic stellate cells proliferation. Zymography showed that curcumin had no effect on matrix metalloproteinase-2 activity. Conclusions: Curcumin inhibited the development of TAA-induced liver cirrhosis mainly due to its anti-inflammatory activities and not by a direct anti-fibrotic effect. As curcumin ingestion is safe in humans, it may be reasonable to assess in clinical studies the beneficial effect of curcumin in slowing the development of liver cirrhosis. © 2007 Blackwell Munksgaard.
Scientific Publication
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