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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Genetic improvement of heat tolerance in Heterorhabditis bacteriophora through hybridization
Year:
1997
Source of publication :
biological control (source)
Authors :
גלזר, איתמר
;
.
שפירו, דוד
;
.
Volume :
8
Co-Authors:
Shapiro, D.I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glazer, I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Segal, D., Dept. of Molec. Microbiol./Biotech., Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
153
To page:
159
(
Total pages:
7
)
Abstract:
A trait for heat tolerance was transferred from the IS5 strain to the HP88 strain of Heterorhabditis bacteriophora Poinar. The transfer was accomplished by allowing the heat tolerant strain (IS5) to mate with the commercial strain (HP88). The hybrid nature of the progeny was confirmed using a marker mutant of the HP88 strain (Hp-dpy-2) and by backcrossing. Progeny from the cross were screened for heat tolerance by measuring survival after 2 h exposure to 40°C. After three and six passages through Galleria mellonella (L.), survival of hybrid nematodes was significantly greater than survival of the HP88 strain and was similar to the survival of the IS5 strain. Fitness, in terms of virulence, reproduction and storage capacity was compared among the IS5, hybrid, and HP88 strains. At 32°C, the IS5 and hybrid strains caused mortality of G. mellonella at a faster rate than the HP88 strain. Similar to the IS5 strain, the hybrids exhibited sensitivity to cold. After 1 week of storage at 10°C, the survival of the hybrid and IS5 strains was significantly reduced relative to the HP88 strain. No differences were detected in reproductive potential among the strains. This study demonstrates the potential of using hybridization to genetically improve entomopathogenic nematodes and illustrates the advantages of using marker mutations in this endeavor.
Note:
Related Files :
biological control
Entomopathogenic
heat tolerance
Heterorhabditidae
hybridization
nematode
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/bcon.1996.0488
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:

Itamar's own PDF.

ID:
32562
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:10
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Scientific Publication
Genetic improvement of heat tolerance in Heterorhabditis bacteriophora through hybridization
8
Shapiro, D.I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Glazer, I., Department of Nematology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Segal, D., Dept. of Molec. Microbiol./Biotech., Tel Aviv University, Tel Aviv 69978, Israel
Genetic improvement of heat tolerance in Heterorhabditis bacteriophora through hybridization
A trait for heat tolerance was transferred from the IS5 strain to the HP88 strain of Heterorhabditis bacteriophora Poinar. The transfer was accomplished by allowing the heat tolerant strain (IS5) to mate with the commercial strain (HP88). The hybrid nature of the progeny was confirmed using a marker mutant of the HP88 strain (Hp-dpy-2) and by backcrossing. Progeny from the cross were screened for heat tolerance by measuring survival after 2 h exposure to 40°C. After three and six passages through Galleria mellonella (L.), survival of hybrid nematodes was significantly greater than survival of the HP88 strain and was similar to the survival of the IS5 strain. Fitness, in terms of virulence, reproduction and storage capacity was compared among the IS5, hybrid, and HP88 strains. At 32°C, the IS5 and hybrid strains caused mortality of G. mellonella at a faster rate than the HP88 strain. Similar to the IS5 strain, the hybrids exhibited sensitivity to cold. After 1 week of storage at 10°C, the survival of the hybrid and IS5 strains was significantly reduced relative to the HP88 strain. No differences were detected in reproductive potential among the strains. This study demonstrates the potential of using hybridization to genetically improve entomopathogenic nematodes and illustrates the advantages of using marker mutations in this endeavor.
Scientific Publication
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