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פותח על ידי קלירמאש פתרונות בע"מ -
Spatial and temporal distribution of pheromone biosynthesis‐activating neuropeptide in Helicoverpa (Heliothis) armigera using RIA and in vitro bioassay
Year:
1991
Authors :
סורוקר, ויקטוריה
;
.
רפאלי, עדה
;
.
Volume :
18
Co-Authors:
Rafaeli, A., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Hirsch, J., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Soroker, V., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Kamensky, B., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Raina, A.K., Insect Neurobiology and Hormone Laboratory, USDA, ARS, Beltsville, Maryland, United States
Facilitators :
From page:
119
To page:
129
(
Total pages:
11
)
Abstract:
A [3H]‐PBAN (pheromone biosynthesis‐activating neuropeptide) analog was synthesized, and binding of the radioligand to a specific PBAN‐antiserum was achieved. The inhibition of binding of the radioligand by unlabeled PBAN, several PBAN analogs, and other competitors was studied and a specific radioimmunoassay was developed. Using this radioimmunoassay we found PBAN‐like immunoreactivity in methanol extracts of hemolymph and neural tissues from females. Higher levels of PBAN‐like immunoreactivity in extracts of brain‐suboesophageal ganglion complexes, corpora cardiaca, thoracic ganglia, and abdominal ganglia were observed during the 4‐5th h scotophase when compared to the PBAN‐like immunoreactivity levels during the 6–11th h photophase. On the other hand, the concentrations of PBAN‐like immunoreactivity in the terminal abdominal ganglion were higher during the photophase relative to minimal levels observed during the scotophase, indicating an accumulation before the onset of pheromone production. These differences in concentrations of PBAN were also reflected in the stimulation of in vitro pheromone glands, whereby significant stimulations were obtained by scotophase and photophase brain extracts, scotophase thoracic ganglia extracts, and photophase terminal abdominal ganglia extracts. No detectable levels of PBAN were found in hemolymph extracts during the sampling periods. Copyright © 1991 Wiley‐Liss, Inc.
Note:
Related Files :
Animal
biosynthesis
Female
metabolism
molecular genetics
Moths
neuropeptide
עוד תגיות
תוכן קשור
More details
DOI :
10.1002/arch.940180206
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32587
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
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Scientific Publication
Spatial and temporal distribution of pheromone biosynthesis‐activating neuropeptide in Helicoverpa (Heliothis) armigera using RIA and in vitro bioassay
18
Rafaeli, A., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Hirsch, J., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Soroker, V., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Kamensky, B., Hebrew University, Department of Entomology, Faculty of Agriculture, Rehovot, Israel
Raina, A.K., Insect Neurobiology and Hormone Laboratory, USDA, ARS, Beltsville, Maryland, United States
Spatial and temporal distribution of pheromone biosynthesis‐activating neuropeptide in Helicoverpa (Heliothis) armigera using RIA and in vitro bioassay
A [3H]‐PBAN (pheromone biosynthesis‐activating neuropeptide) analog was synthesized, and binding of the radioligand to a specific PBAN‐antiserum was achieved. The inhibition of binding of the radioligand by unlabeled PBAN, several PBAN analogs, and other competitors was studied and a specific radioimmunoassay was developed. Using this radioimmunoassay we found PBAN‐like immunoreactivity in methanol extracts of hemolymph and neural tissues from females. Higher levels of PBAN‐like immunoreactivity in extracts of brain‐suboesophageal ganglion complexes, corpora cardiaca, thoracic ganglia, and abdominal ganglia were observed during the 4‐5th h scotophase when compared to the PBAN‐like immunoreactivity levels during the 6–11th h photophase. On the other hand, the concentrations of PBAN‐like immunoreactivity in the terminal abdominal ganglion were higher during the photophase relative to minimal levels observed during the scotophase, indicating an accumulation before the onset of pheromone production. These differences in concentrations of PBAN were also reflected in the stimulation of in vitro pheromone glands, whereby significant stimulations were obtained by scotophase and photophase brain extracts, scotophase thoracic ganglia extracts, and photophase terminal abdominal ganglia extracts. No detectable levels of PBAN were found in hemolymph extracts during the sampling periods. Copyright © 1991 Wiley‐Liss, Inc.
Scientific Publication
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