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פותח על ידי קלירמאש פתרונות בע"מ -
Studies on growth inhibition by lectins of penicillia and aspergilli - 1978
Year:
1978
Source of publication :
Archives of Microbiology
Authors :
ברקאי-גולן, רבקה
;
.
Volume :
116
Co-Authors:

Barkai-Golan, R., Department of Biophysics, The Weizmann Institute of Science, Rehovoth, Israel
Mirelman, D., Department of Biophysics, The Weizmann Institute of Science, Rehovoth, Israel
Sharon, N., Department of Biophysics, The Weizmann Institute of Science, Rehovoth, Israel

Facilitators :
From page:
119
To page:
124
(
Total pages:
6
)
Abstract:
It has previously been shown in our laboratory that wheat germ agglutinin (WGA) binds to Trichoderma viride and inhibits growth of this fungus. Here we report on the effect of WGA, soybean agglutinin (SBA) and peanut agglutinin (PNA) on Penicillia and Aspergilli. Binding of the lectins to the fungi was examined with the aid of their fluorescein isothiocyanate (FITC) conjugated derivatives. FITC-WGA bound to young hyphal walls of all species, in particular to the hyphal tips and septa, in agreement with the chitinous composition of the cell walls of the two genera. Hyphae of all species examined were labelled, though in different patterns, by FITC-SBA and FITC-PNA, suggesting the presence of galactose residues on their surfaces. Young conidiophores, metulae (of the Penicillia), vesicles (of the Aspergilli), sterigmata and young spores, were also labelled. The three lectins inhibited incorporation of [3H]acetate, N-acetyl-D-[3H]glucosamine and D-[14C]galactose into young hyphae of Aspergillus ochraceus, indicating interference with fungal growth. Inhibition of spore germination by the three lectins was also observed. Preincubation of the lectins with their specific saccharide inhibitors prevented binding and the inhibitory effects. We conclude that lectins are useful tools for the study of fungal cell surfaces, and may also serve as an important aid in fungal classification. The present findings also support the suggestion that one role of lectins in plants is protection against fungal pathogens. © 1978 Springer-Verlag.
Note:
Related Files :
animal experiment
Aspergillus
aspergillus ochraceus
Fungal cell walls
fungi
Penicillium
Penicillium italicum
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00406026
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32588
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
Scientific Publication
Studies on growth inhibition by lectins of penicillia and aspergilli - 1978
116

Barkai-Golan, R., Department of Biophysics, The Weizmann Institute of Science, Rehovoth, Israel
Mirelman, D., Department of Biophysics, The Weizmann Institute of Science, Rehovoth, Israel
Sharon, N., Department of Biophysics, The Weizmann Institute of Science, Rehovoth, Israel

Studies on growth inhibition by lectins of penicillia and aspergilli
It has previously been shown in our laboratory that wheat germ agglutinin (WGA) binds to Trichoderma viride and inhibits growth of this fungus. Here we report on the effect of WGA, soybean agglutinin (SBA) and peanut agglutinin (PNA) on Penicillia and Aspergilli. Binding of the lectins to the fungi was examined with the aid of their fluorescein isothiocyanate (FITC) conjugated derivatives. FITC-WGA bound to young hyphal walls of all species, in particular to the hyphal tips and septa, in agreement with the chitinous composition of the cell walls of the two genera. Hyphae of all species examined were labelled, though in different patterns, by FITC-SBA and FITC-PNA, suggesting the presence of galactose residues on their surfaces. Young conidiophores, metulae (of the Penicillia), vesicles (of the Aspergilli), sterigmata and young spores, were also labelled. The three lectins inhibited incorporation of [3H]acetate, N-acetyl-D-[3H]glucosamine and D-[14C]galactose into young hyphae of Aspergillus ochraceus, indicating interference with fungal growth. Inhibition of spore germination by the three lectins was also observed. Preincubation of the lectins with their specific saccharide inhibitors prevented binding and the inhibitory effects. We conclude that lectins are useful tools for the study of fungal cell surfaces, and may also serve as an important aid in fungal classification. The present findings also support the suggestion that one role of lectins in plants is protection against fungal pathogens. © 1978 Springer-Verlag.
Scientific Publication
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