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פותח על ידי קלירמאש פתרונות בע"מ -
A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression
Year:
1992
Source of publication :
Molecular and Cellular Biology
Authors :
שיקלר, מיכאל
;
.
Volume :
12
Co-Authors:
Schickler, M., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
Lira, S.A., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
Kinloch, R.A., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
Wassarman, P.M., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
Facilitators :
From page:
120
To page:
127
(
Total pages:
8
)
Abstract:
The gene encoding mZP3, the mouse sperm receptor, is expressed exclusively in growing oocytes during oogenesis. To investigate the molecular basis of oocyte-specific mZP3 gene expression, we generated several lines of mice harboring a transgene that contains 470 bp of mZP3 gene 5'-flanking sequence (nucleotides -470 to +10) fused to the firefly luciferase gene coding region. Three of four expressing transgenic lines exhibited luciferase activity only in growing oocytes, suggesting that the 470-bp fragment is sufficient to direct oocyte-specific expression of the luciferase gene. Results of DNase I footprinting and gel mobility shift assays suggested the presence of an ovary-specific protein that binds to a small region (nucleotides -99 to -86) within the 470-bp fragment of the mZP3 promoter, with 5'-G(G/A)T(G/A)A-3' representing the minimal sequence required for binding. Southwestern (DNA- protein) gel blots revealed the presence of an oocyte-specific, ~60,000-M(r) protein, called OSP-1, that binds to the minimal sequence. Changes in levels of OSP-1 during oogenesis and early cleavage are consistent with the pattern of mZP3 gene expression during these developmental stages in mice. Therefore, OSP-1 may be a mammalian oocyte-specific transcription factor involved in regulating oocyte-specific mZP3 gene expression.
Note:
Related Files :
Animal
animal experiment
DNA
DNA binding protein
gene expression
Mammalia
mice
Plasmid
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32640
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
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A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression
12
Schickler, M., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
Lira, S.A., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
Kinloch, R.A., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
Wassarman, P.M., Cell/Developmental Biol. Dept., Roche Molecular Biology Inst., Roche Research Center, Nutley, NJ 07110, United States
A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression
The gene encoding mZP3, the mouse sperm receptor, is expressed exclusively in growing oocytes during oogenesis. To investigate the molecular basis of oocyte-specific mZP3 gene expression, we generated several lines of mice harboring a transgene that contains 470 bp of mZP3 gene 5'-flanking sequence (nucleotides -470 to +10) fused to the firefly luciferase gene coding region. Three of four expressing transgenic lines exhibited luciferase activity only in growing oocytes, suggesting that the 470-bp fragment is sufficient to direct oocyte-specific expression of the luciferase gene. Results of DNase I footprinting and gel mobility shift assays suggested the presence of an ovary-specific protein that binds to a small region (nucleotides -99 to -86) within the 470-bp fragment of the mZP3 promoter, with 5'-G(G/A)T(G/A)A-3' representing the minimal sequence required for binding. Southwestern (DNA- protein) gel blots revealed the presence of an oocyte-specific, ~60,000-M(r) protein, called OSP-1, that binds to the minimal sequence. Changes in levels of OSP-1 during oogenesis and early cleavage are consistent with the pattern of mZP3 gene expression during these developmental stages in mice. Therefore, OSP-1 may be a mammalian oocyte-specific transcription factor involved in regulating oocyte-specific mZP3 gene expression.
Scientific Publication
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