נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Early genes induced in hepatic stellate cells during wound healing
Year:
1997
Source of publication :
Gene
Authors :
לאלזר, אברהם
;
.
Volume :
195
Co-Authors:
Lalazar, A., ARO Volcani Center, Bet Dagan, Israel
Wong, L., Med. Serv. and USCF Liver Ctr. Lab., Bldg. 40, San Francisco General Hospital, 1001 Potrero Ave., San Francisco, CA 94110, United States
Yamasaki, G., Med. Serv. and USCF Liver Ctr. Lab., Bldg. 40, San Francisco General Hospital, 1001 Potrero Ave., San Francisco, CA 94110, United States
Friedman, S.L., Med. Serv. and USCF Liver Ctr. Lab., Bldg. 40, San Francisco General Hospital, 1001 Potrero Ave., San Francisco, CA 94110, United States
Facilitators :
From page:
235
To page:
243
(
Total pages:
9
)
Abstract:
Activation of mesenchymal cells is a central event in the wound healing response of most tissues. In liver, the mesenchymal element responsible for organ fibrosis is the hepatic stellate cell (HSC) (formerly known as lipocyte or Ito cell). The phenotypic cascade of stellate cell activation in liver fibrosis has been well documented and involves both marked morphologic changes and upregulation of several functional components including extracellular matrix, cytokine receptors, contractile filaments and metalloproteinases. However, the genetic regulation of stellate cell activation is poorly understood. In an attempt to clone genes that are involved in the regulation of HSC activation we have combined cDNA library amplification by PCR with subtraction hybridization/differential screening, and have successfully identified genes induced in vivo during early stellate cell activation in a rat model of liver fibrosis. The subtracted cDNA library comprised less than 100 unique sequences. Of these, 13 clones with sizes ranging from 322 to 745 were sequenced and characterized. Gene induction in HSCs was monitored by RNAse protection assay during early liver injury induced by the hepatotoxin CCl4. The sequenced cDNAs corresponding to the known genes included type II transforming growth factor β receptor, glutathione peroxidase I, transferrin and several clones encoding cellular retrotransposons, whose expression was not previously identified in non-parenchymal liver cells. In addition, one partial cDNA predicted a zinc-finger motif, suggesting a possible role of a novel transcriptional regulator. Our approach represents a valuable strategy for clarifying in vivo regulatory mechanisms of mesenchymal cell activation in wound healing.
Note:
Related Files :
animal cell
animal experiment
Animals
gene amplification
Male
phenotype
stellate cell
transferrin
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0378-1119(97)00159-5
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32655
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
You may also be interested in
Scientific Publication
Early genes induced in hepatic stellate cells during wound healing
195
Lalazar, A., ARO Volcani Center, Bet Dagan, Israel
Wong, L., Med. Serv. and USCF Liver Ctr. Lab., Bldg. 40, San Francisco General Hospital, 1001 Potrero Ave., San Francisco, CA 94110, United States
Yamasaki, G., Med. Serv. and USCF Liver Ctr. Lab., Bldg. 40, San Francisco General Hospital, 1001 Potrero Ave., San Francisco, CA 94110, United States
Friedman, S.L., Med. Serv. and USCF Liver Ctr. Lab., Bldg. 40, San Francisco General Hospital, 1001 Potrero Ave., San Francisco, CA 94110, United States
Early genes induced in hepatic stellate cells during wound healing
Activation of mesenchymal cells is a central event in the wound healing response of most tissues. In liver, the mesenchymal element responsible for organ fibrosis is the hepatic stellate cell (HSC) (formerly known as lipocyte or Ito cell). The phenotypic cascade of stellate cell activation in liver fibrosis has been well documented and involves both marked morphologic changes and upregulation of several functional components including extracellular matrix, cytokine receptors, contractile filaments and metalloproteinases. However, the genetic regulation of stellate cell activation is poorly understood. In an attempt to clone genes that are involved in the regulation of HSC activation we have combined cDNA library amplification by PCR with subtraction hybridization/differential screening, and have successfully identified genes induced in vivo during early stellate cell activation in a rat model of liver fibrosis. The subtracted cDNA library comprised less than 100 unique sequences. Of these, 13 clones with sizes ranging from 322 to 745 were sequenced and characterized. Gene induction in HSCs was monitored by RNAse protection assay during early liver injury induced by the hepatotoxin CCl4. The sequenced cDNAs corresponding to the known genes included type II transforming growth factor β receptor, glutathione peroxidase I, transferrin and several clones encoding cellular retrotransposons, whose expression was not previously identified in non-parenchymal liver cells. In addition, one partial cDNA predicted a zinc-finger motif, suggesting a possible role of a novel transcriptional regulator. Our approach represents a valuable strategy for clarifying in vivo regulatory mechanisms of mesenchymal cell activation in wound healing.
Scientific Publication
You may also be interested in