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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea
Year:
1999
Authors :
אלעד, יגאל
;
.
Volume :
105
Co-Authors:
Elad, Y., Department of Plant Pathology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Kapat, A., Department of Plant Pathology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
177
To page:
189
(
Total pages:
13
)
Abstract:
The role of protease of Trichoderma harzianum in the biocontrol of Botrytis cinema was examined. Two isolates of T. harzianum were compared for their ability to produce protease in liquid culture medium and on the surface of bean leaves. The biocontrol agent T. harziaum T39 produced 58 mU/ml of protease and T. harzianum NCIM1185 produced 54 mU/ml on the 5th day of growth in liquid culture medium. On bean leaves, combinations of B. cinema and T. harzianum isolates were examined for the synthesis of protease. The protease activities were 0.9 and 0.6 mU/ml for T. harzianum T39 and NCIM1185, respectively, and 0.5 mU/ml for B. cinema alone after 48 h of incubation. In the presence of T. harzianum T39 culture liquid containing protease, a 55% reduction in B. cinema germination and a 80% reduction in the germ tube length were observed after 17 h of incubation in vitro. When T. harzianum isolates were added to B. cinema on bean leaves, increased synthesis of protease was observed (1.0 and 1.2 mU/ml for T39 and NCIM1185, respectively). In the presence of T. harzianum NCIM1185 protease, although the rate of germination was reduced, B. cinerea attained 98% germination after 17h of incubation. The hydrolytic enzymes produced by B. cinerea, endo-polygalacturonase (PG) and exoPG were partially deactivated by protease from the T. harzianum isolates. Carboxymethyl cellulase was deactivated only by protease of NCIM1185. On the surface of bean leaves, the protease (obtained from liquid culture medium of T. harzianum isolates) resulted in 56-100% reduction of disease severity. The culture liquid containing protease synthesized on the surface of bean leaves treated with B. cinema and with T. harzianum was collected and added to fresh leaves infected by B. cinema. There was 56-100% and 30-75% reduction of disease severity with liquid droplet collected from the leaves treated with T. harzianum T39 and NCIM1185, respectively. Increased control of disease was obtained by combining the conidia of T. harzianum isolates with protease obtained from culture media. Protease inhibitors, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E64), antipain hydrochloride, and a mixture of inhibitors, but not pepstatin A, fully or partially nullified the biocontrol effect of T39. T39 was found to be a poor producer of chitinase and β-1,3-glucanase in vitro. These enzymes were not detected on leaves treated with T39. Involvement of protease in biocontrol of B. cinema is suggested.
Note:
Related Files :
biological control
carboxymethyl cellulase
Grey mould
Mechanisms
Pathogenicity enzymes
Polygalacturonase
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/A:1008753629207
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32661
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
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Scientific Publication
The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea
105
Elad, Y., Department of Plant Pathology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
Kapat, A., Department of Plant Pathology, Institute of Plant Protection, Volcani Center, Bet Dagan 50250, Israel
The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea
The role of protease of Trichoderma harzianum in the biocontrol of Botrytis cinema was examined. Two isolates of T. harzianum were compared for their ability to produce protease in liquid culture medium and on the surface of bean leaves. The biocontrol agent T. harziaum T39 produced 58 mU/ml of protease and T. harzianum NCIM1185 produced 54 mU/ml on the 5th day of growth in liquid culture medium. On bean leaves, combinations of B. cinema and T. harzianum isolates were examined for the synthesis of protease. The protease activities were 0.9 and 0.6 mU/ml for T. harzianum T39 and NCIM1185, respectively, and 0.5 mU/ml for B. cinema alone after 48 h of incubation. In the presence of T. harzianum T39 culture liquid containing protease, a 55% reduction in B. cinema germination and a 80% reduction in the germ tube length were observed after 17 h of incubation in vitro. When T. harzianum isolates were added to B. cinema on bean leaves, increased synthesis of protease was observed (1.0 and 1.2 mU/ml for T39 and NCIM1185, respectively). In the presence of T. harzianum NCIM1185 protease, although the rate of germination was reduced, B. cinerea attained 98% germination after 17h of incubation. The hydrolytic enzymes produced by B. cinerea, endo-polygalacturonase (PG) and exoPG were partially deactivated by protease from the T. harzianum isolates. Carboxymethyl cellulase was deactivated only by protease of NCIM1185. On the surface of bean leaves, the protease (obtained from liquid culture medium of T. harzianum isolates) resulted in 56-100% reduction of disease severity. The culture liquid containing protease synthesized on the surface of bean leaves treated with B. cinema and with T. harzianum was collected and added to fresh leaves infected by B. cinema. There was 56-100% and 30-75% reduction of disease severity with liquid droplet collected from the leaves treated with T. harzianum T39 and NCIM1185, respectively. Increased control of disease was obtained by combining the conidia of T. harzianum isolates with protease obtained from culture media. Protease inhibitors, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E64), antipain hydrochloride, and a mixture of inhibitors, but not pepstatin A, fully or partially nullified the biocontrol effect of T39. T39 was found to be a poor producer of chitinase and β-1,3-glucanase in vitro. These enzymes were not detected on leaves treated with T39. Involvement of protease in biocontrol of B. cinema is suggested.
Scientific Publication
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