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פותח על ידי קלירמאש פתרונות בע"מ -
Spatial learning induced changes in expression of the ryanodine type II receptor in the rat hippocampus
Year:
2000
Source of publication :
FASEB Journal
Authors :
מאירי, נעם
;
.
Volume :
14
Co-Authors:
Zhao, W., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States, Laboratory of Adaptive Systems, NINDS, National Institutes of Health, 36 Convent Dr., Bethesda, MD 20892, United States
Meiri, N., Institute of Animal Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Xu, H., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States
Cavallaro, S., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States, Inst. Bioimaging Pathophysiology C., CNR Catania, Italy, Oasi Inst. Res. Mental Retard. B., Troina (EN), Italy
Quattrone, A., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States
Zhang, L., Behavioral Endocrinology Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, United States
Alkon, D.L., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States
Facilitators :
From page:
290
To page:
300
(
Total pages:
11
)
Abstract:
Calcium signaling critical to neural functions is mediated through Ca2+ channels localized on both the plasma membrane and intracellular organelles such as endoplasmic reticulum. Whereas Ca2+ influx occurs via the voltage- or/and ligand-sensitive Ca2+ channels, Ca2+ release from intracellular stores that amplifies further the Ca2+ signal is thought to be involved in more profound and lasting changes in neurons. The ryanodine receptor, one of the two major intracellular Ca2+ channels, has been an important target for studying Ca2+ signaling in brain functions, including learning and memory, due to its characteristic Ca2+-induced Ca2+ release. In this study, we report regional and cellular distributions of the type-2 ryanodine receptor (RyR2) mRNA in the rat brain, and effects of spatial learning on RyR2 gene expression at mRNA and protein levels in the rat hippocampus. Using in situ hybridization, reverse transcription polymerase chain reaction, and ribonuclease protection assays, significant increases in RyR2 mRNA were found in the hippocampus of rats trained in an intensive water maze task. With immunoprecipitation and immunoblotting, protein levels of RyR2 were also demonstrated to be increased in the microsomal fractions prepared from hippocampi of trained rats. These results suggest that RyR2, and hence the RyR2-mediated Ca2+ signals, may be involved in memory processing after spatial learning. The increases in RyR2 mRNA and protein at 12 and 24 h after training could contribute to more permanent changes such as structural modifications during long-term memory storage.
Note:
Related Files :
animal experiment
animal model
Animals
animal tissue
immunoprecipitation
Male
Ryanodine Receptor Calcium Release Channel
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32662
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
You may also be interested in
Scientific Publication
Spatial learning induced changes in expression of the ryanodine type II receptor in the rat hippocampus
14
Zhao, W., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States, Laboratory of Adaptive Systems, NINDS, National Institutes of Health, 36 Convent Dr., Bethesda, MD 20892, United States
Meiri, N., Institute of Animal Science, ARO, Volcani Center, Bet Dagan 50250, Israel
Xu, H., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States
Cavallaro, S., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States, Inst. Bioimaging Pathophysiology C., CNR Catania, Italy, Oasi Inst. Res. Mental Retard. B., Troina (EN), Italy
Quattrone, A., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States
Zhang, L., Behavioral Endocrinology Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, United States
Alkon, D.L., Laboratory of Adaptive Systems, Natl. Inst. Neurol. Disord. Stroke, National Institutes of Health, Bethesda, MD 20892, United States
Spatial learning induced changes in expression of the ryanodine type II receptor in the rat hippocampus
Calcium signaling critical to neural functions is mediated through Ca2+ channels localized on both the plasma membrane and intracellular organelles such as endoplasmic reticulum. Whereas Ca2+ influx occurs via the voltage- or/and ligand-sensitive Ca2+ channels, Ca2+ release from intracellular stores that amplifies further the Ca2+ signal is thought to be involved in more profound and lasting changes in neurons. The ryanodine receptor, one of the two major intracellular Ca2+ channels, has been an important target for studying Ca2+ signaling in brain functions, including learning and memory, due to its characteristic Ca2+-induced Ca2+ release. In this study, we report regional and cellular distributions of the type-2 ryanodine receptor (RyR2) mRNA in the rat brain, and effects of spatial learning on RyR2 gene expression at mRNA and protein levels in the rat hippocampus. Using in situ hybridization, reverse transcription polymerase chain reaction, and ribonuclease protection assays, significant increases in RyR2 mRNA were found in the hippocampus of rats trained in an intensive water maze task. With immunoprecipitation and immunoblotting, protein levels of RyR2 were also demonstrated to be increased in the microsomal fractions prepared from hippocampi of trained rats. These results suggest that RyR2, and hence the RyR2-mediated Ca2+ signals, may be involved in memory processing after spatial learning. The increases in RyR2 mRNA and protein at 12 and 24 h after training could contribute to more permanent changes such as structural modifications during long-term memory storage.
Scientific Publication
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