אסיף מאגר המחקר החקלאי

Culture of newly isolated tobacco protoplasts: cell division and precursor incorporation following a transient exposure to coumarin

Year:

1980

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Volume :

18

Co-Authors:

Zelcer, A., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Galun, E., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel

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Abstract:

The first nuclear divisions in tobacco mesophyll protoplasts were detected 16-20 h after the beginning of culture in a chemically defined medium and 80-90% of the cells devided at least once during the first 40-44 h of culture. This onset of cell division as well as the concomitant acceleration of the rates of thymidine, uridine and leucine incorporations were inhibited by 250 mg/l coumarin. When newly isolated protoplasts were exposed for 20 h to coumarin and then transferred to a medium devoid of coumarin a fast initiation of cell division and an immediate commencement of pre cursor incorporation was observed. These findings have implications for the use of coumarin for the synchronization of cultured, newly isolated, plant protoplasts. © 1980.

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