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פותח על ידי קלירמאש פתרונות בע"מ -
Culture of newly isolated tobacco protoplasts: cell division and precursor incorporation following a transient exposure to coumarin
Year:
1980
Source of publication :
Plant Science Letters
Authors :
זלצר, אהרון
;
.
Volume :
18
Co-Authors:
Zelcer, A., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Galun, E., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
185
To page:
190
(
Total pages:
6
)
Abstract:
The first nuclear divisions in tobacco mesophyll protoplasts were detected 16-20 h after the beginning of culture in a chemically defined medium and 80-90% of the cells devided at least once during the first 40-44 h of culture. This onset of cell division as well as the concomitant acceleration of the rates of thymidine, uridine and leucine incorporations were inhibited by 250 mg/l coumarin. When newly isolated protoplasts were exposed for 20 h to coumarin and then transferred to a medium devoid of coumarin a fast initiation of cell division and an immediate commencement of pre cursor incorporation was observed. These findings have implications for the use of coumarin for the synchronization of cultured, newly isolated, plant protoplasts. © 1980.
Note:
Related Files :
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0304-4211(80)90049-8
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32684
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
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Scientific Publication
Culture of newly isolated tobacco protoplasts: cell division and precursor incorporation following a transient exposure to coumarin
18
Zelcer, A., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Galun, E., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Culture of newly isolated tobacco protoplasts: cell division and precursor incorporation following a transient exposure to coumarin
The first nuclear divisions in tobacco mesophyll protoplasts were detected 16-20 h after the beginning of culture in a chemically defined medium and 80-90% of the cells devided at least once during the first 40-44 h of culture. This onset of cell division as well as the concomitant acceleration of the rates of thymidine, uridine and leucine incorporations were inhibited by 250 mg/l coumarin. When newly isolated protoplasts were exposed for 20 h to coumarin and then transferred to a medium devoid of coumarin a fast initiation of cell division and an immediate commencement of pre cursor incorporation was observed. These findings have implications for the use of coumarin for the synchronization of cultured, newly isolated, plant protoplasts. © 1980.
Scientific Publication
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