נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Citrus chlorophyllase dynamics at ethylene-induced fruit color-break: A study of chlorophyllase expression, posttranslational processing kinetics, and in situ intracellular localization
Year:
2008
Authors :
אזולאי, תמר
;
.
איל, יורם
;
.
בלאוסוב, אדוארד
;
.
Volume :
148
Co-Authors:
Shemer, T.A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Harpaz-Saad, S., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Lovat, N., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Krokhin, O., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Spicer, V., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Standing, K.G., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Goldschmidt, E.E., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Eyal, Y., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Facilitators :
From page:
108
To page:
118
(
Total pages:
11
)
Abstract:
Fruit color-break is the visual manifestation of the developmentally regulated transition of chloroplasts to chromoplasts during fruit ripening and often involves biosynthesis of copious amounts of carotenoids concomitant with massive breakdown of chlorophyll. Regulation of chlorophyll breakdown at different physiological and developmental stages of the plant life cycle, particularly at fruit color-break, is still not well understood. Here, we present the dynamics of native chlorophyllase (Chlase) and chlorophyll breakdown in lemon (Citrus limon) fruit during ethylene-induced color-break. We show, using in situ immunofluorescence on ethylene-treated fruit peel (flavedo) tissue, that citrus Chlase is located in the plastid, in contrast to recent reports suggesting cytoplasmic localization of Arabidopsis (Arabidopsis thaliana) Chlases. At the intra-organellar level, Chlase signal was found to overlap mostly with chlorophyll fluorescence, suggesting association of most of the Chlase protein with the photosynthetic membranes. Confocal microscopy analysis showed that the kinetics of chlorophyll breakdown was not uniform in the flavedo cells. Chlorophyll quantity at the cellular level was negatively correlated with plastid Chlase accumulation; plastids with reduced chlorophyll content were found by in situ immunofluorescence to contain significant levels of Chlase, while plastids containing still-intact chlorophyll lacked any Chlase signal. Immunoblot and protein-mass spectrometry analyses were used to demonstrate that citrus Chlase initially accumulates as an approximately 35-kD precursor, which is subsequently N-terminally processed to approximately 33-kD mature forms by cleavage at either of three consecutive amino acid positions. Chlase plastid localization, expression kinetics, and the negative correlation with chlorophyll levels support the central role of the enzyme in chlorophyll breakdown during citrus fruit color-break. © 2008 American Society of Plant Biologists.
Note:
Related Files :
arabidopsis
Arabidopsis thaliana
chlorophyll
chlorophyllase
Citrus limon
ethylene
metabolism
עוד תגיות
תוכן קשור
More details
DOI :
10.1104/pp.108.124933
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32695
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:11
You may also be interested in
Scientific Publication
Citrus chlorophyllase dynamics at ethylene-induced fruit color-break: A study of chlorophyllase expression, posttranslational processing kinetics, and in situ intracellular localization
148
Shemer, T.A., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Harpaz-Saad, S., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel, R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Belausov, E., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Lovat, N., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Krokhin, O., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Spicer, V., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Standing, K.G., Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB R3E 3P4, Canada
Goldschmidt, E.E., R.H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Eyal, Y., Institute of Plant Sciences, Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel
Citrus chlorophyllase dynamics at ethylene-induced fruit color-break: A study of chlorophyllase expression, posttranslational processing kinetics, and in situ intracellular localization
Fruit color-break is the visual manifestation of the developmentally regulated transition of chloroplasts to chromoplasts during fruit ripening and often involves biosynthesis of copious amounts of carotenoids concomitant with massive breakdown of chlorophyll. Regulation of chlorophyll breakdown at different physiological and developmental stages of the plant life cycle, particularly at fruit color-break, is still not well understood. Here, we present the dynamics of native chlorophyllase (Chlase) and chlorophyll breakdown in lemon (Citrus limon) fruit during ethylene-induced color-break. We show, using in situ immunofluorescence on ethylene-treated fruit peel (flavedo) tissue, that citrus Chlase is located in the plastid, in contrast to recent reports suggesting cytoplasmic localization of Arabidopsis (Arabidopsis thaliana) Chlases. At the intra-organellar level, Chlase signal was found to overlap mostly with chlorophyll fluorescence, suggesting association of most of the Chlase protein with the photosynthetic membranes. Confocal microscopy analysis showed that the kinetics of chlorophyll breakdown was not uniform in the flavedo cells. Chlorophyll quantity at the cellular level was negatively correlated with plastid Chlase accumulation; plastids with reduced chlorophyll content were found by in situ immunofluorescence to contain significant levels of Chlase, while plastids containing still-intact chlorophyll lacked any Chlase signal. Immunoblot and protein-mass spectrometry analyses were used to demonstrate that citrus Chlase initially accumulates as an approximately 35-kD precursor, which is subsequently N-terminally processed to approximately 33-kD mature forms by cleavage at either of three consecutive amino acid positions. Chlase plastid localization, expression kinetics, and the negative correlation with chlorophyll levels support the central role of the enzyme in chlorophyll breakdown during citrus fruit color-break. © 2008 American Society of Plant Biologists.
Scientific Publication
You may also be interested in