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BMC Plant Biology
Brandi, F., Consiglio per la Ricerca in Agricoltura, Unità di Ricerca per la Frutticoltura-Forlì (CRA-FRF), via la Canapona 1 bis, 47100 Forlì, Italy
Bar, E., Dept. of Vegetable Crops, ARO Newe Ya'ar Research Center, P.O. Box 1021, 30095 Ramat Yishay, Israel
Mourgues, F., National Agency for New technologies, Energy and Sustainable Economic Development (ENEA), Trisaia Research Center, S.S. 106 km 419+500, 75026 Rotondella, Italy
Horváth, G., University of Pécs, Medical School Department of Pharmacognosy, H-7624 Pécs, Rókus u. 2, Hungary
Turcsi, E., University of Pécs, Medical School, Department of Biochemistry and Medical Chemistry, H-7624 Pécs, Szigeti út 12, Hungary
Giuliano, G., ENEA, Casaccia Research Center, Via Anguillarese 301, 00123 Roma, Italy
Liverani, A., Consiglio per la Ricerca in Agricoltura, Unità di Ricerca per la Frutticoltura-Forlì (CRA-FRF), via la Canapona 1 bis, 47100 Forlì, Italy
Tartarini, S., Dipartimento Colture Arboree, Università di Bologna, via Fanin 42, 40127 Bologna, Italy
Lewinsohn, E., Dept. of Vegetable Crops, ARO Newe Ya'ar Research Center, P.O. Box 1021, 30095 Ramat Yishay, Israel
Rosati, C., National Agency for New technologies, Energy and Sustainable Economic Development (ENEA), Trisaia Research Center, S.S. 106 km 419+500, 75026 Rotondella, Italy
Background: Carotenoids are plant metabolites which are not only essential in photosynthesis but also important quality factors in determining the pigmentation and aroma of flowers and fruits. To investigate the regulation of carotenoid metabolism, as related to norisoprenoids and other volatile compounds in peach (Prunus persica L. Batsch.), and the role of carotenoid dioxygenases in determining differences in flesh color phenotype and volatile composition, the expression patterns of relevant carotenoid genes and metabolites were studied during fruit development along with volatile compound content. Two contrasted cultivars, the yellow-fleshed 'Redhaven' (RH) and its white-fleshed mutant 'Redhaven Bianca' (RHB) were examined.Results: The two genotypes displayed marked differences in the accumulation of carotenoid pigments in mesocarp tissues. Lower carotenoid levels and higher levels of norisoprenoid volatiles were observed in RHB, which might be explained by differential activity of carotenoid cleavage dioxygenase (CCD) enzymes. In fact, the ccd4 transcript levels were dramatically higher at late ripening stages in RHB with respect to RH. The two genotypes also showed differences in the expression patterns of several carotenoid and isoprenoid transcripts, compatible with a feed-back regulation of these transcripts. Abamine SG - an inhibitor of CCD enzymes - decreased the levels of both isoprenoid and non-isoprenoid volatiles in RHB fruits, indicating a complex regulation of volatile production.Conclusions: Differential expression of ccd4 is likely to be the major determinant in the accumulation of carotenoids and carotenoid-derived volatiles in peach fruit flesh. More in general, dioxygenases appear to be key factors controlling volatile composition in peach fruit, since abamine SG-treated 'Redhaven Bianca' fruits had strongly reduced levels of norisoprenoids and other volatile classes. Comparative functional studies of peach carotenoid cleavage enzymes are required to fully elucidate their role in peach fruit pigmentation and aroma. © 2011 Brandi et al; licensee BioMed Central Ltd.
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תנאי שימוש
Study of 'Redhaven' peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism
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Brandi, F., Consiglio per la Ricerca in Agricoltura, Unità di Ricerca per la Frutticoltura-Forlì (CRA-FRF), via la Canapona 1 bis, 47100 Forlì, Italy
Bar, E., Dept. of Vegetable Crops, ARO Newe Ya'ar Research Center, P.O. Box 1021, 30095 Ramat Yishay, Israel
Mourgues, F., National Agency for New technologies, Energy and Sustainable Economic Development (ENEA), Trisaia Research Center, S.S. 106 km 419+500, 75026 Rotondella, Italy
Horváth, G., University of Pécs, Medical School Department of Pharmacognosy, H-7624 Pécs, Rókus u. 2, Hungary
Turcsi, E., University of Pécs, Medical School, Department of Biochemistry and Medical Chemistry, H-7624 Pécs, Szigeti út 12, Hungary
Giuliano, G., ENEA, Casaccia Research Center, Via Anguillarese 301, 00123 Roma, Italy
Liverani, A., Consiglio per la Ricerca in Agricoltura, Unità di Ricerca per la Frutticoltura-Forlì (CRA-FRF), via la Canapona 1 bis, 47100 Forlì, Italy
Tartarini, S., Dipartimento Colture Arboree, Università di Bologna, via Fanin 42, 40127 Bologna, Italy
Lewinsohn, E., Dept. of Vegetable Crops, ARO Newe Ya'ar Research Center, P.O. Box 1021, 30095 Ramat Yishay, Israel
Rosati, C., National Agency for New technologies, Energy and Sustainable Economic Development (ENEA), Trisaia Research Center, S.S. 106 km 419+500, 75026 Rotondella, Italy
Study of 'Redhaven' peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism
Background: Carotenoids are plant metabolites which are not only essential in photosynthesis but also important quality factors in determining the pigmentation and aroma of flowers and fruits. To investigate the regulation of carotenoid metabolism, as related to norisoprenoids and other volatile compounds in peach (Prunus persica L. Batsch.), and the role of carotenoid dioxygenases in determining differences in flesh color phenotype and volatile composition, the expression patterns of relevant carotenoid genes and metabolites were studied during fruit development along with volatile compound content. Two contrasted cultivars, the yellow-fleshed 'Redhaven' (RH) and its white-fleshed mutant 'Redhaven Bianca' (RHB) were examined.Results: The two genotypes displayed marked differences in the accumulation of carotenoid pigments in mesocarp tissues. Lower carotenoid levels and higher levels of norisoprenoid volatiles were observed in RHB, which might be explained by differential activity of carotenoid cleavage dioxygenase (CCD) enzymes. In fact, the ccd4 transcript levels were dramatically higher at late ripening stages in RHB with respect to RH. The two genotypes also showed differences in the expression patterns of several carotenoid and isoprenoid transcripts, compatible with a feed-back regulation of these transcripts. Abamine SG - an inhibitor of CCD enzymes - decreased the levels of both isoprenoid and non-isoprenoid volatiles in RHB fruits, indicating a complex regulation of volatile production.Conclusions: Differential expression of ccd4 is likely to be the major determinant in the accumulation of carotenoids and carotenoid-derived volatiles in peach fruit flesh. More in general, dioxygenases appear to be key factors controlling volatile composition in peach fruit, since abamine SG-treated 'Redhaven Bianca' fruits had strongly reduced levels of norisoprenoids and other volatile classes. Comparative functional studies of peach carotenoid cleavage enzymes are required to fully elucidate their role in peach fruit pigmentation and aroma. © 2011 Brandi et al; licensee BioMed Central Ltd.
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