חיפוש מתקדם
Phytopathologia Mediterranea
Yurtmen, M., Plant Protection Research Institute, Kisla Caddesi, PK 21, 01321, Adana, Turkey
Spiegel, S., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Maslenin, L., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Yilmaz, M.A., The University of Cukurova, Faculty of Agriculture, Plant Protection Department, 01330, Adana, Turkey
Rosner, A., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
The applicability of single and double-stranded transcript conformation polymorphism (TCP) for differentiation among Prunus necrotic ringspot virus (PNRSV) isolates was evaluated and compared with other molecular differentiation procedures. Single-stranded TCP was found to be suitable to differentiate closely related virus isolates. However, due to its high variability, it was not suitable for grouping virus isolates. Double-stranded TCP, on the other hand, enabled the division of virus isolates into major groups. Restriction fragment length polymorphism (RFLP) of the PCR products confirmed the grouping of the virus isolates but this technique was limited in its ability to detect a wide range of nucleotide modifications. Nucleotide sequence analysis was essential for the detection of strain-specific sequences but did not clearly identify most other minor modifications that are necessary for virus classification. The combination of all methods is therefore sometimes required for complete analysis.
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הספר "אוצר וולקני"
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תנאי שימוש
Assessing the applicability of transcript conformation polymorphism for differentiation among Prunus necrotic ringspot virus isolates
41
Yurtmen, M., Plant Protection Research Institute, Kisla Caddesi, PK 21, 01321, Adana, Turkey
Spiegel, S., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Maslenin, L., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Yilmaz, M.A., The University of Cukurova, Faculty of Agriculture, Plant Protection Department, 01330, Adana, Turkey
Rosner, A., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Assessing the applicability of transcript conformation polymorphism for differentiation among Prunus necrotic ringspot virus isolates
The applicability of single and double-stranded transcript conformation polymorphism (TCP) for differentiation among Prunus necrotic ringspot virus (PNRSV) isolates was evaluated and compared with other molecular differentiation procedures. Single-stranded TCP was found to be suitable to differentiate closely related virus isolates. However, due to its high variability, it was not suitable for grouping virus isolates. Double-stranded TCP, on the other hand, enabled the division of virus isolates into major groups. Restriction fragment length polymorphism (RFLP) of the PCR products confirmed the grouping of the virus isolates but this technique was limited in its ability to detect a wide range of nucleotide modifications. Nucleotide sequence analysis was essential for the detection of strain-specific sequences but did not clearly identify most other minor modifications that are necessary for virus classification. The combination of all methods is therefore sometimes required for complete analysis.
Scientific Publication
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