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פותח על ידי קלירמאש פתרונות בע"מ -
Assessing the applicability of transcript conformation polymorphism for differentiation among Prunus necrotic ringspot virus isolates
Year:
2002
Source of publication :
Phytopathologia Mediterranea
Authors :
מסלנין, לודמילה
;
.
רוזנר, אריה
;
.
שפיגל, שרה
;
.
Volume :
41
Co-Authors:
Yurtmen, M., Plant Protection Research Institute, Kisla Caddesi, PK 21, 01321, Adana, Turkey
Spiegel, S., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Maslenin, L., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Yilmaz, M.A., The University of Cukurova, Faculty of Agriculture, Plant Protection Department, 01330, Adana, Turkey
Rosner, A., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Facilitators :
From page:
92
To page:
98
(
Total pages:
7
)
Abstract:
The applicability of single and double-stranded transcript conformation polymorphism (TCP) for differentiation among Prunus necrotic ringspot virus (PNRSV) isolates was evaluated and compared with other molecular differentiation procedures. Single-stranded TCP was found to be suitable to differentiate closely related virus isolates. However, due to its high variability, it was not suitable for grouping virus isolates. Double-stranded TCP, on the other hand, enabled the division of virus isolates into major groups. Restriction fragment length polymorphism (RFLP) of the PCR products confirmed the grouping of the virus isolates but this technique was limited in its ability to detect a wide range of nucleotide modifications. Nucleotide sequence analysis was essential for the detection of strain-specific sequences but did not clearly identify most other minor modifications that are necessary for virus classification. The combination of all methods is therefore sometimes required for complete analysis.
Note:
Related Files :
PNRS
Prunus
Prunus necrotic ringspot virus
RFLP
Strain differentiation
TCP
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32738
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:12
You may also be interested in
Scientific Publication
Assessing the applicability of transcript conformation polymorphism for differentiation among Prunus necrotic ringspot virus isolates
41
Yurtmen, M., Plant Protection Research Institute, Kisla Caddesi, PK 21, 01321, Adana, Turkey
Spiegel, S., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Maslenin, L., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Yilmaz, M.A., The University of Cukurova, Faculty of Agriculture, Plant Protection Department, 01330, Adana, Turkey
Rosner, A., Virology Department, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel
Assessing the applicability of transcript conformation polymorphism for differentiation among Prunus necrotic ringspot virus isolates
The applicability of single and double-stranded transcript conformation polymorphism (TCP) for differentiation among Prunus necrotic ringspot virus (PNRSV) isolates was evaluated and compared with other molecular differentiation procedures. Single-stranded TCP was found to be suitable to differentiate closely related virus isolates. However, due to its high variability, it was not suitable for grouping virus isolates. Double-stranded TCP, on the other hand, enabled the division of virus isolates into major groups. Restriction fragment length polymorphism (RFLP) of the PCR products confirmed the grouping of the virus isolates but this technique was limited in its ability to detect a wide range of nucleotide modifications. Nucleotide sequence analysis was essential for the detection of strain-specific sequences but did not clearly identify most other minor modifications that are necessary for virus classification. The combination of all methods is therefore sometimes required for complete analysis.
Scientific Publication
You may also be interested in