Co-Authors:
Dov Kishinevsky, B., Department of Agronomy and Natural Resources, ARO, Volcani Center, Bet Dagan, 50250, Israel
Sen, D., Department of Soil and Crop Sciences, Texas A and M University, College Station, TX 77843, United States
Yang, G., S. Tolkowsky Laboratory, ARO, Volcani Center, Bet Dagan, 50250, Israel
Abstract:
Cultural and physiological properties, serology, plasmid profiles and infective traits were determined for 23 strains of rhizobia isolated from various Hedysarum species: H. coronarium (common name: sulla) (16), H. carnosum (1), H. alpinum (3), H. mackenzii (2) and H. pallens (1) from Portugal, Spain Tunisia Alaska and Israel. Strains isolated from H. alpinum, H. mackenzii and H. pallens have slow growth rates on yeast-extract mannitol medium and were unable to nodulate H. coronarium plants, whereas the latter were effectively nodulated by all sixteen fast growing strains from sulla. Regardless of the country of origin all H. coronarium strains fell into one serogroup and were not serologically related with strains of other Hedysarum species. The RAPD (random amplified polymorphic DNA) fingerprinting method which was carried out on five H. coronarium and three H. alpinum strains allowed distinction to be made among serologically related rhizobia. No particular plasmid profile pattern was observed in relation to the host or geographical origin of the strains.